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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In Silico, In Vitro, and In Vivo Antitumor and Anti-Inflammatory Evaluation of a Standardized Alkaloid-Enriched Fraction Obtained fromBoehmeria caudataSw. Aerial Parts

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de Paiva, Paula P. [1, 2, 3] ; Nunes, Julia H. B. [4, 3] ; Nonato, Fabiana R. [3] ; Ruiz, Ana L. T. G. [2, 3] ; Zafred, Rafael R. T. [1, 3] ; Sousa, Ilza M. O. [2, 3] ; Okubo, Marcia Y. [3, 5] ; Kawano, Daniel F. [2] ; Monteiro, Paula A. [1, 3] ; Foglio, Mary A. [2] ; Carvalho, Joao E. [1, 2]
Total Authors: 11
[1] Univ Campinas UNICAMP, Inst Biol, BR-13083862 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Fac Pharmaceut Sci, BR-13083871 Campinas, SP - Brazil
[3] Univ Campinas UNICAMP, Chem Biol & Agr Pluridisciplinary Res Ctr CPQBA, BR-13148218 Paulinia, SP - Brazil
[4] Univ Campinas UNICAMP, Inst Chem, BR-13083970 Campinas, SP - Brazil
[5] Univ Estadual Campinas, Piracicaba Dent Sch, UNICAMP, BR-13414903 Piracicaba, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Molecules; v. 25, n. 17 SEP 2020.
Web of Science Citations: 0

In the context of the cancer-inflammation relationship and the use of natural products as potential antitumor and anti-inflammatory agents, the alkaloid-enriched fraction ofBoehmeriacaudata(BcAEF) aerial parts was evaluated. In vitro antiproliferative studies with human tumor cell lines showed high activity at low concentrations. Further investigation on NCI-H460 cells showed an irreversible effect on cell proliferation, with cell cycle arrest at G2/M phase and programmed cell death induction. Molecular docking studies of four alkaloids identified in BcAEF with colchicine's binding site on beta-tubulin were performed, suggesting (-)-C (15R)-hydroxycryptopleurine as the main inductor of the observed mitotic death. In vivo studies showed that BcAEF was able to reduce Ehrlich tumor volume progression by 30 to 40%. Checking myeloperoxidase activity, BcAEF reduced neutrophils migration towards the tumor. The in vivo anti-inflammatory activity was evaluated by chemically induced edema models. In croton oil-induced ear edema and carrageenan (CG)-induced paw edema models, BcAEF reduced edema around 70 to 80% together with inhibition of activation and/or migration of neutrophils to the inflammatory area. All together the results presented herein show BcAEF as a potent antitumor agent combining antiproliferative and anti-inflammatory properties, which could be further explored in (pre)clinical studies. (AU)

FAPESP's process: 17/21801-2 - Predictors of severity and new treatments for bone marrow neoplasias
Grantee:Sara Teresinha Olalla Saad
Support type: Research Projects - Thematic Grants
FAPESP's process: 12/19661-4 - Vouacapans and geranilgeraniol synergic effect evaluation on nociceptive pain, pharmacokinetic and preclinical toxicological studies
Grantee:Mary Ann Foglio
Support type: Regular Research Grants
FAPESP's process: 11/22458-3 - Isolation, identification and mechanism of action of fractions and active principles of Boehmeria caudata Sw with anticancer activity.
Grantee:Paula Pereira de Paiva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/06636-7 - In vitro antiproliferative activity of Coronarin-D
Grantee:Márcia Yumi Okubo
Support type: Scholarships in Brazil - Master
FAPESP's process: 11/22457-7 - Isolation, identification and mechanism of action of anticancer active principles of Croton campestris A.St.-Hil.
Grantee:Paula Araujo Monteiro
Support type: Scholarships in Brazil - Doctorate