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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of nanoemulsion modification with chitosan and sodium alginate on the topical delivery and efficacy of the cytotoxic agent piplartine in 2D and 3D skin cancer models

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Giacone, V, Daniela ; Dartora, Vanessa F. M. C. [1] ; de Matos, Jenyffer K. R. [1] ; Passos, Julia S. [1] ; Miranda, Daniel A. G. [1] ; de Oliveira, Erica A. [2] ; Silveira, Edilberto R. [3] ; Costa-Lotufo, V, Leticia ; Maria-Engler, Silvya S. [2] ; Lopes, Luciana B. [4]
Total Authors: 10
[1] Giacone, Daniela, V, Univ Sao Paulo, Inst Biomed Sci, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci, Sao Paulo, SP - Brazil
[3] Univ Fed Ceara, Dept Inorgan & Organ Chem, Fortaleza, Ceara - Brazil
[4] Costa-Lotufo, Leticia, V, Giacone, Daniela, V, Univ Sao Paulo, Inst Biomed Sci, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: International Journal of Biological Macromolecules; v. 165, n. A, p. 1055-1065, DEC 15 2020.
Web of Science Citations: 2

Due to the limited options for topical management of skin cancer, this study aimed at developing and evaluating nanoemulsions (NE) for topical delivery of the cytotoxic agent piplartine (piperlongumine). NEs were modified with chitosan or sodium alginate, and the effects on the physicochemical properties, piplartine delivery and formulation efficacy were evaluated. The nanoemulsion droplets displayed similar size (96-112 nm), but opposite charge; the polysaccharides improved piplartine penetration into and across the skin (1.3-1.9-fold) in a similar manner, increasing the ratio ``drug in the skin/receptor phase{''} by 1.4-1.5-fold compared to the plain NE and highlighting their relevance for cutaneous localization. Oleic acid addition to the chitosan-containing NE further increased drug penetration (similar to 1.9-2.0-fold), as did increases in drug content from 0.5 to 1%. The cytotoxicity of piplartine was similar to 2.8-fold higher when the drug was incorporated in the chitosan-containing NE compared to its solution (IC50 = 14.6 mu M) against melanoma cells. The effects of this nanocarrier on 3D melanoma tissues were concentration-related; at 1%, piplartine elicited marked epidermis destruction. These results support the potential applicability of the chitosan-modified nanoemulsion containing piplartine as a new strategy for local management of skin cancer. (C) 2020 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 16/16554-3 - Emerging genes in melanoma progression and chemoresistance
Grantee:Érica Aparecida de Oliveira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/04174-4 - Development and evaluation of cationic bioadhesive nanocarriers as a platform to localize piplartine in the breast for tumor treatment
Grantee:Vanessa Franco Carvalho Dartora
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 18/18813-1 - Bioresponsive gels containing nanoparticles for exudate control and co-delivery of propranolol and adenosine for wound healing applications
Grantee:Jenyffer Kelly Rocha de Matos
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 18/13877-1 - Nanocarriers for localized treatment and chemoprevention of breast tumors
Grantee:Luciana Biagini Lopes
Support type: Research Grants - Young Investigators Grants - Phase 2
FAPESP's process: 17/07856-9 - Cationic nanoemulsions for piplartine cutaneous delivery and treatment of skin tumors
Grantee:Daniela Veronesi Giacone
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 20/01208-8 - Bioadhesive nanostructured systems for intraductal administration and localized treatment of Breast Cancer
Grantee:Julia Sapienza Passos
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 15/17177-6 - Integrative approach on the sustainable prospection of marine natural products: from diversity to anticancer compounds
Grantee:Leticia Veras Costa Lotufo
Support type: BIOTA-FAPESP Program - Thematic Grants