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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Synthesis of multifunctional chlorhexidine-doped thin films for titanium-based implant materials

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Matos, Adaias Oliveira [1, 2] ; de Almeida, Amanda Bandeira [1] ; Beline, Thamara [1] ; Tonon, Caroline C. [3] ; Viana Casarin, Renato Correa [1] ; Windsor, Lester Jack [2] ; Duarte, Simone [3] ; Nociti Jr, Francisco Humberto ; Rangel, Elidiane Cipriano [4] ; Gregory, Richard L. [2] ; Ricardo Barao, Valentim Adelino [5]
Total Authors: 11
[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, Piracicaba, SP - Brazil
[2] Indiana Univ, Sch Dent, Dept Biomed Sci & Comprehens Care, Indianapolis, IN - USA
[3] Indiana Univ, Sch Dent, Purdue Univ Indianapolis, Dept Cariol Operat Dent & Dent Publ Hlth, Indianapolis, IN - USA
[4] Sao Paulo State Univ UNESP, Sci & Technol Inst Sorocaba ICTS, Lab Technol Plasmas LaPTec, Sorocaba, SP - Brazil
[5] Nociti Jr, Jr., Francisco Humberto, Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, Piracicaba, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Materials Science & Engineering C-Materials for Biological Applications; v. 117, DEC 2020.
Web of Science Citations: 0

Our goal was to create bio-functional chlorhexidine (CHX)-doped thin films on commercially pure titanium (cpTi) discs using the glow discharge plasma approach. Different plasma deposition times (50, 35 and 20 min) were used to create bio-functional surfaces based on silicon films with CHX that were compared to the control groups {[}no CHX and bulk cpTi surface (machined)]. Physico-chemical and biological characterizations included: 1. Morphology, roughness, elemental chemical composition, film thickness, contact angle and surface free energy; 2. CHX-release rate; 3. Antibacterial effect on Streptococcus sanguinis biofilms at 24, 48 and 72 h; 4. Cytotoxicity and metabolic activity using fibroblasts cell culture (NIH-F3T3 cells) at 1, 2, 3 and 4 days; 5. Protein expression by NIH-F3T3 cells at 1, 2, 3 and 4 days; and 6. Co-culture assay of fibroblasts cells and S. sanguinis to assess live and dead cells on the confocal laser scanning microscopy, mitochondrial activity (XTT), membrane leakage (LDH release), and metabolic activity (WST-1 assay) at 1, 2 and 3 days of co-incubation. Data analysis showed that silicon films, with or without CHX coated cpTi discs, increased surface wettability and free energy (p < 0.05) without affecting surface roughness. CHX release was maintained over a 22-day period and resulted in a significant inhibition of biofilm growth (p 0.05) at 48 and 72 h of biofilm formation for 50 min and 20 min of plasma deposition time groups, respectively. In general, CHX treatment did not significantly affect NIH-F3T3 cell viability (p 0.05). Co-culture demonstrated that CHX-doped film did not affect the metabolic activity, cytotoxicity and viability of fibroblasts cells (p 0.05). Altogether, the findings of the current study support the conclusion that silicon films added with CHX can be successfully created on titanium discs and have the potential to affect bacterial growth and inflammatory markers without affecting cell viability/proliferation rates. (AU)

FAPESP's process: 16/06117-5 - Development of hexamethyldisiloxane films incorporated with chlorhexidine onto titanium surface: antimicrobial potential and cytotoxicity
Grantee:Adaias Oliveira Matos
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/21894-0 - Antimicrobial and biocompatibility potentials of hexamethyldisiloxane films incorporated with chlorhexidine on titanium surface using a co-infected in vitro reconstituted gingival oral epithelium model
Grantee:Adaias Oliveira Matos
Support type: Scholarships abroad - Research Internship - Doctorate