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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Luciferase isozymes from the Brazilian Aspisoma lineatum (Lampyridae) firefly: origin of efficient pH-sensitive lantern luciferases from fat body pH-insensitive ancestors

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Carvalho, M. C. [1] ; Tomazini, A. [2] ; Amaral, D. T. [3] ; Murakami, M. T. [2] ; Viviani, V. R. [1, 3]
Total Authors: 5
[1] Fed Univ Sao Carlos UFSCAR, Grad Program Evolut Genet & Mol Biol, Sao Carlos, SP - Brazil
[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas, SP - Brazil
[3] Fed Univ Sao Carlos UFSCar, Grad Program Biotechnol & Environm Monitoring, Sorocaba - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 19, n. 12, p. 1750-1764, DEC 1 2020.
Web of Science Citations: 2

The incorporation of photochromic dyes into porous metal-organic frameworks (MOFs) is an attractive way to transfer the photochromic properties of the dye to a solid crystalline material. In this work, the well-known P-type chromophore 1,2-bis{[}2-methylbenzo{[}b]thiophen-3-yl]-3,3,4,4,5,5-hexafluoro-1-cycl opentene (DTE) is embedded in three different MOFs, namely MOF-5, MIL-68(In), and MIL-68(Ga). The successful filling of the MOF pores with the DTE guest was proven by X-ray powder diffraction, while the amount of the embedded guest molecules was investigated by X-ray photoelectron spectroscopy (XPS), liquid-state NMR and thermal analysis (DSC/TGA). The measurements reveal an unexpectedly low filling of the MOF pores with the DTE guest (e.g. in MOF-5 only every fifth MOF pore is filled with a guest molecule) as well as an inhomogeneous loading throughout the material. Reflection spectra clearly show the transitions of the colourless open-ring and the coloured closed-ring forms of the DTE guest upon UV (lambda = 365 nm), blue (lambda = 405 nm) and green (lambda = 535 nm) light exposure, where the latter is usually suppressed in crystalline DTE. Remarkably, no fatigue after ten switching cycles was observed and a high thermal stability of the coloured closed-ring form (at 50 degrees C for 1 h) was achieved. (AU)

FAPESP's process: 10/05426-8 - Arthropod bioluminescence: biological diversity in Brazilian biomes, biochemical origin, structural/functional evolution of luciferases, molecular differentiation of lanterns, biotechnological, environmental and educational applications
Grantee:Vadim Viviani
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/07312-6 - Structure and biological function of luciferase-like enzime of Arachnocampa luminosa lanterns (Diptera: Keroplatidae) and luminescent activity development by genetic engineering
Grantee:Mariele Cristina de Carvalho
Support type: Scholarships in Brazil - Doctorate (Direct)