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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cyclophosphamide modulated the foreign body inflammatory reaction in tilapia (Oreochromis niloticus)

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Charlie-Silva, Ives [1] ; Conde, Gabriel [2] ; Mendonca Gomes, Juliana Moreira [1] ; da Rosa Prado, Ed Johnny [2] ; Fernandes, Dayanne Carla [2] ; de Moraes, Alessandra Cristina [3] ; Eto, Silas Fernandes [4] ; Conceicao, Katia [5] ; de Andrade Belo, Marco Antonio [2, 6]
Total Authors: 9
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo, SP - Brazil
[2] Sao Paulo State Univ UNESP, Dept Prevent Vet Med, Jaboticabal, SP - Brazil
[3] Fed Univ Rondonia UNIR, Vet Coll, Rolim De Moura, RO - Brazil
[4] Fed Univ Roraima UFRR, Dept Grad Program Hlth Sci, Boa Vista, PR - Brazil
[5] UNIFESP, Lab Bioquim Peptideos, Sao Jose Dos Campos, SP - Brazil
[6] Brasil Univ, Lab Anim Pharmacol & Toxicol, Av Hilario da Silva Passos, 950 Jardim Univ, BR-13690000 Descalvado, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: FISH & SHELLFISH IMMUNOLOGY; v. 107, n. A, p. 230-237, DEC 2020.
Web of Science Citations: 0

In order to understand events and mechanisms present in the pathophysiology of tilapia's chronic inflammation and based on the immunomodulatory activity attributed to cyclophosphamide which is widely used to suppress immune responses in human medicine, the present study investigated the effects of cyclophosphamide (CYP) treatment on the modulation of foreign body inflammatory reaction in Nile tilapia (Oreochromis niloticus) with round glass coverslip implanted in the subcutaneous tissue (9 mm of diameter). Forty tilapia (151 +/- 10,2 g) were randomly distributed in 5 aquariums (n = 8) with a capacity of 250 L of water each, to compose two treatments (sampled 3 and 6 days post-implantation): implanted/untreated (control) and implanted/treated with 200 mg of CYP kg(-1) of b.w., through i.p. route. A fifth group (n = 8) was sampled without any stimulus (naive) to obtain reference values. CYP-treated tilapia showed decrease in macrophage accumulation, giant cell formation and Langhans cells on the glass coverslip when compared to control fish. The treatment with CYP resulted in decrease of leukocyte and thrombocyte counts. Decrease in alpha-2-macroglobulin, ceruloplasmin, albumin and transferrin levels, as well as increase in haptoglobin, complement C3 and apolipoprotein A1 were observed in tilapias during foreign body inflammation. Blood levels of complement C3, alpha-2-macroglobulin, ceruloplasmin and transferrin were modulated by treatment with CYP. Therefore, the treatment with 200 mg of CYP kg(-1) of b.w. in tilapia resulted in an anti-inflammatory effect by suppressing the dynamics between leukocytes in the bloodstream and macrophage accumulation with giant cell formation in the inflamed focus, as well as by modulating APPs during foreign body reaction. (AU)

FAPESP's process: 13/11675-9 - Therapeutic efficacy study, clinical and ecotoxicological safety of the Fosfomicine antibiotic, administered by oral, for Aeromonas hydrophila and Streptococcus agalactiae treatment in Nile Tilapia, Oreochromis niloticus
Grantee:Roberto Barbuio
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/12256-7 - Study of the involvement of insulin and exogenous glucocorticoid on acute inflammatory response of Nile tilapia, Oreochromis niloticus, challenged with Aeromonas hydrophila.
Grantee:Marco Antonio de Andrade Belo
Support type: Regular Research Grants