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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Histoplasma capsulatum chemotypes I and II induce IL-8 secretion in lung epithelial cells in distinct manners

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Alcantara, Cristiane [1] ; Almeida, Bruna Rocha [1] ; Silva Campitelli Barros, Bianca Carla [1] ; Orikaza, Cristina Mary [1] ; Toledo, Marcos Sergio [2] ; Suzuki, Erika [1]
Total Authors: 6
[1] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Escola Paulista Med, Sao Paulo, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Biochem, Escola Paulista Med, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Medical Mycology; v. 58, n. 8, p. 1169-1177, NOV 2020.
Web of Science Citations: 1

The cell wall is one of the most important structures of pathogenic fungi, enabling initial interaction with the host and consequent modulation of immunological responses. Over the years, some researchers have shown that cell wall components of Histoplasma capsulatum vary among fungal isolates, and one of the major differences is the presence or absence of alpha-(1,3)-glucan, classifying wild-type fungi as chemotypes II or I, respectively. The present work shows that an isolate of H. capsulatum chemotype I induced lower levels of interleukin (IL)-8 secretion by the lung epithelial cell line A549, when compared to chemotype II yeasts. Thus, we expected that the absence of alpha-glucan in spontaneous variant yeasts, which were isolated from chemotype II cultures, would modify IL-8 secretion by A549 cells, but surprisingly, these fungi promoted similar levels of IL-8 secretion as their wild-type counterpart. Furthermore, when using a specific inhibitor for Syk activation, we observed that this inhibitor reduced IL-8 levels in A549 cell cultures infected with wild type chemotype I fungi. This inhibitor failed to reduce this cytokine levels in A549 cell cultures infected with chemotype II and their spontaneous variant yeasts, which also do not present alpha-glucan on their surface. The importance of SFKs and PKC delta in this event was also analyzed. Our results show that different isolates of H. capsulatum modulate distinct cell signaling pathways to promote cytokine secretion in host epithelial cells, emphasizing the existence of various mechanisms for Histoplasma pathogenicity. (AU)

FAPESP's process: 15/25652-6 - Study of the communication between receptors, and their signaling pathways, in fungus-induced cytokine secretion in epithelial cells
Grantee:Erika Suzuki de Toledo
Support Opportunities: Regular Research Grants
FAPESP's process: 16/06285-5 - Involvement of toll-like receptor, integrins and their respective cell signaling pathways in cytokine secretion by epithelial cells during infection with Paracoccidioides brasiliensis
Grantee:Bianca Carla Silva Campitelli de Barros
Support Opportunities: Scholarships in Brazil - Doctorate