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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Human Papillomavirus DNA Detection by Droplet Digital PCR in Formalin-Fixed Paraffin-Embedded Tumor Tissue from Oropharyngeal Squamous Cell Carcinoma Patients

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Schiavetto, Camila Marques [1] ; de Abreu, Priscila Marinho [2] ; von Zeidler, Sandra Ventorin [2] ; de Jesus, Lais Machado [1] ; Carvalho, Raiany Santos [3] ; Cirino, Maria Thereza [1] ; Carloni, Adriana Cruvinel [1] ; Oliveira, Cristina [1] ; Scapulatempo-Neto, Cristovam [1, 4] ; de Almeida, Gisele Caravina [5, 4] ; de Menezes, Nei Soares [5] ; Carvalho, Andre Lopes [1] ; Reis, Rui Manuel [6, 7, 1] ; de Carvalho, Ana Carolina [1]
Total Authors: 14
[1] Barretos Canc Hosp, Mol Oncol Res Ctr, Rua Antenor Duarte Vilela 1331, BR-14784400 Barretos, SP - Brazil
[2] Univ Fed Espirito Santo, Programa Posgrad Biotecnol, Ctr Ciencias Saude, Vitoria, ES - Brazil
[3] Barretos Canc Hosp, Res Support Ctr, Sao Paulo - Brazil
[4] Diagnost Amer DASA, Pathol & Mol Diagnost Serv, Sao Paulo - Brazil
[5] Barretos Canc Hosp, Dept Pathol, Sao Paulo - Brazil
[6] ICVS 3Bs PT Govt Associate Lab, Braga - Portugal
[7] Univ Minho, Sch Med, Life & Hlth Sci Res Inst ICVS, Braga - Portugal
Total Affiliations: 7
Document type: Journal article
Source: MOLECULAR DIAGNOSIS & THERAPY; v. 25, n. 1 NOV 2020.
Web of Science Citations: 0

Introduction High-risk human papillomavirus infection impacts staging and prognosis of oropharyngeal squamous cell carcinomas (OPSCCs). Determination of HPV status in tumor tissue by p16-immunohistochemistry (p16-IHC) can be challenging; therefore, complementary methodologies could be useful in a clinical setting. Objective To test for accuracy and clinical relevance of HPV-DNA detection in formalin-fixed and paraffin-embedded (FFPE) tumor samples by droplet digital PCR (ddPCR). Materials and Methods Fifty OPSCCs were tested for p16-IHC status followed by HPV-16/18 DNA detection/quantification in FFPE-recovered DNA using ddPCR. Accuracy for HPV status determination and association with patient information were also evaluated. Results 32.0% (16/50) of the cases were p16-IHC positive (p16 +), 42.0% (21/50) had detectable levels of HPV-16 DNA, and none were positive for HPV-18 DNA. A higher median viral load of HPV-16 DNA was observed in p16 + cases (p < 0.0001). Concordance between p16-IHC and HPV-16 DNA ranged from 78.0 to 86.0% and accuracy rates were between 78.0 and 86.0%. P16-IHC and HPV-16 DNA detection was associated with gender, smoking status, and tumor subsite, while only HPV-16 DNA was associated with cT stage. The combination of HPV positivity by p16-IHC and ddPCR showed higher overall survival rates in comparison with p16 + /HPV-DNA- and p16 - /HPV-DNA- results. Conclusions Type-specific HPV-DNA detection by ddPCR is highly specific but moderately sensitive for the determination of HPV status and showed clinical relevance, mainly when associated with p16-IHC status. Results highlight the importance of performing HPV-DNA testing in combination with p16-IHC for proper identification of HPV-associated OPSCC and to improve clinical management of OPSCC patients. (AU)

FAPESP's process: 15/01286-0 - Characterization of genetic and epigenetic alterations in specific genes in HPV-positive and HPV-negative tumors of patients with head and neck squamous cell carcinoma
Grantee:André Lopes Carvalho
Support type: Regular Research Grants