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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Novel biotechnological approaches for monitoring and immunization against resistant to antibiotics Escherichia coli and other pathogenic bacteria

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Belizario, Jose E. [1] ; Sircili, Marcelo P. [2]
Total Authors: 2
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Av Lineu Prestes 1524, BR-05508900 Sao Paulo, SP - Brazil
[2] Butantan Inst, Genet Lab, Av Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BMC Veterinary Research; v. 16, n. 1 NOV 2 2020.
Web of Science Citations: 0

The application of next-generation molecular, biochemical and immunological methods for developing new vaccines, antimicrobial compounds, probiotics and prebiotics for zoonotic infection control has been fundamental to the understanding and preservation of the symbiotic relationship between animals and humans. With increasing rates of antibiotic use, resistant bacterial infections have become more difficult to diagnose, treat, and eradicate, thereby elevating the importance of surveillance and prevention programs. Effective surveillance relies on the availability of rapid, cost-effective methods to monitor pathogenic bacterial isolates. In this opinion article, we summarize the results of some research program initiatives for the improvement of live vaccines against avian enterotoxigenic Escherichia coli using virulence factor gene deletion and engineered vaccine vectors based on probiotics. We also describe methods for the detection of pathogenic bacterial strains in eco-environmental headspace and aerosols, as well as samples of animal and human breath, based on the composition of volatile organic compounds and fatty acid methyl esters. We explain how the introduction of these low-cost biotechnologies and protocols will provide the opportunity to enhance co-operation between networks of resistance surveillance programs and integrated routine workflows of veterinary and clinical public health microbiology laboratories. (AU)

FAPESP's process: 12/02497-7 - Expression analysis of genes regulated by protein Dermicidina in malignant melanoma cells G-361 by DNA microarray
Grantee:Nancy Marcela Pérez Sosa
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/20847-0 - Site-specific incorporation of non-natural amino acids in proteins: development of new incorporation technologies and the use in the study of proteins
Grantee:Vitor Marcelo Silveira Bueno Brandão de Oliveira
Support Opportunities: Regular Research Grants
FAPESP's process: 10/06707-0 - Role of LuxS in the regulation of virulence factors in human and animal enteropathogenic Escherichia coli.
Grantee:Vanessa Bueris
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 08/05519-6 - Quorum sensing in Escherichia coli
Grantee:Marcelo Palma Sircili
Support Opportunities: Regular Research Grants