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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunological and bacteriological shifts associated with a flagellin-hyperproducing Salmonella Enteritidis mutant in chickens

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Author(s):
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Barbosa, Fernanda de Oliveira [1] ; de Freitas Neto, Oliveiro Caetano [2] ; Rodrigues Alves, Lucas Bocchini [1] ; Benevides, Valdinete Pereira [1] ; Secundo de Souza, Andrei Itajahy [1] ; Rubio, Marcela da Silva [1] ; de Almeida, Adriana Maria [1] ; Saraiva, Mauro Mesquita [1] ; Bruno de Oliveira, Celso Jose [3] ; Olsen, John Elmerdahl [4] ; Berchieri Junior, Angelo [1]
Total Authors: 11
Affiliation:
[1] State Univ Sao Paulo, Sch Agr & Veterinarian Sci FCAV, Dept Vet Pathol, Jaboticabal - Brazil
[2] Fed Univ Minas Gerais UFMG, Vet Sch, Dept Prevent Vet Med, Av Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG - Brazil
[3] Fed Univ Paraiba CCA UFPB, Ctr Agr Sci, Dept Anim Sci, Areia, PB - Brazil
[4] Univ Copenhagen, Dept Vet & Anim Sci, Stigbojlen 4, DK-1870 Frederiksberg C - Denmark
Total Affiliations: 4
Document type: Journal article
Source: Brazilian Journal of Microbiology; v. 52, n. 1, SI NOV 2020.
Web of Science Citations: 0
Abstract

Salmonella Enteritidis causes infections in humans and animals which are often associated with extensive gut colonization and bacterial shedding in faeces. The natural presence of flagella in Salmonella enterica has been shown to be enough to induce pro-inflammatory responses in the gut, resulting in recruitment of polymorphonuclear cells, gut inflammation and, consequently, reducing the severity of systemic infection in chickens. On the other hand, the absence of flagellin in some Salmonella strains favours systemic infection as a result of the poor intestinal inflammatory responses elicited. The hypothesis that higher production of flagellin by certain Salmonella enterica strains could lead to an even more immunogenic and less pathogenic strain for chickens was here investigated. In the present study, a Salmonella Enteritidis mutant strain harbouring deletions in clpP and fliD genes (SE Delta clpPfliD), which lead to overexpression of flagellin, was generated, and its immunogenicity and pathogenicity were comparatively assessed to the wild type in chickens. Our results showed that SE Delta clpPfliD elicited more intense immune responses in the gut during early stages of infection than the wild type did, and that this correlated with earlier intestinal and systemic clearance of the bacterium. (AU)

FAPESP's process: 18/04883-8 - Study of gene expression in chicken infected with strains of Salmonella enteritidis and Salmonella typhimurium containing deletions in clpP and fliD genes
Grantee:Fernanda de Oliveira Barbosa
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 16/10369-0 - Pathogenicity of Salmonella spp. with deletions in the genes phoPQ, fliD e clpP in comercial poultry
Grantee:Angelo Berchieri Junior
Support type: Regular Research Grants