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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bisphosphonate-based surface biofunctionalization improves titanium biocompatibility

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Albano, Carolina Simao [1, 2] ; Gomes, Anderson Moreira [2] ; Feltran, Georgia da Silva [2] ; da Costa Fernandes, Celio Junior [2] ; Trino, Luciana Daniele [1] ; Zambuzzi, Willian Fernando [3, 2] ; Lisboa-Filho, Paulo Noronha [1]
Total Authors: 7
[1] UNESP Sao Paulo State Univ, Sch Sci, Dept Phys, Bauru, SP - Brazil
[2] UNESP Sao Paulo State Univ, Inst Biosci Botucatu, Dept Chem & Biochem, Botucatu, SP - Brazil
[3] UNESP Sao Paulo State Univ, Electron Microscopy Ctr, Inst Biosci Botucatu, Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 1

Novel-biofunctionalized surfaces are required to improve the performance of endosseous implants, which are mainly related to the resistance against biocorrosion, as well as for the consideration of osteoinductive phenomena. Among different strategies, the use of bisphosphonate molecules as linkers between titanium dioxide (TiO2) surfaces and proteins is a distinctive approach, one in which bisphosphonate could play a role in the osseointegration. Thus, to address this issue, we proposed a novel biofunctionalization of TiO2 surfaces using sodium alendronate (ALN) as a linker and bovine serum albumin as the protein. Physicochemical analysis of the functionalized surfaces was performed using contact angle analyses and surface roughness measurements, which indicated an efficient functionalization. The biocompatibility of the functionalized surfaces was analyzed through the adhesion behavior of the pre-osteoblasts onto the samples. Overall, our data showed a significant improvement concerning the cell adhesion by modulating the adhesion cell-related set of genes. The obtained results show that for modified surfaces there is an increase of up to 100 times in the percentage of cells adhered when compared to the control, besides the extracellular matrix remodeling seemed to be an essential prerequisite for the early stages of cell adhesion on to the biomaterials, which was assayed by evaluating the matrix metalloproteinase activities as well as the gene activations. In the expressions of the Bsp and Bglap2 genes, for the group containing ALN (TiO2 + ALN), it was observed an increase in expression (approximately sixfold change) when compared to the control. Altogether, our data clearly showed that the bisphosphonate-biofunctionalized surface enhanced the biocompatibility of titanium and claims to further progress preclinical in vivo experimentation. (AU)

FAPESP's process: 17/02366-3 - Biocompatibility of functionalized TiO2 thin films
Grantee:Carolina Simão Albano
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/15035-5 - Adsorption of bisphosphonates and proteins on TiO2 surfaces for bone implants
Grantee:Paulo Noronha Lisboa Filho
Support Opportunities: Regular Research Grants
FAPESP's process: 14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration
Grantee:Willian Fernando Zambuzzi
Support Opportunities: Research Grants - Young Investigators Grants