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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

MAMP-triggered Medium Alkalinization of Plant Cell Cultures

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Author(s):
Fiorin, Gabriel L. [1, 2, 3] ; Sanchez-Vallet, Andrea [3, 4] ; Thomma, Bart P. H. J. [3] ; Pereira, Goncalo A. G. [2] ; Teixeira, Paulo J. P. L. [5]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Grad Program Genet & Mol Biol, Campinas - Brazil
[2] Univ Estadual Campinas, Lab Genom & Expressao, Campinas - Brazil
[3] Wageningen Univ & Res, Lab Phytopathol, Wageningen - Netherlands
[4] Swiss Fed Inst Technol, Inst Integrat Biol, Plant Pathol, Zurich - Switzerland
[5] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz ESALQ, Dept Ciencias Biol, Lab Genet & Imunol Plantas, Piracicaba - Brazil
Total Affiliations: 5
Document type: Journal article
Source: BIO-PROTOCOL; v. 10, n. 8 APR 20 2020.
Web of Science Citations: 0
Abstract

Plants recognize a wide variety of microbial molecules to detect and respond to potential invaders. Recognition of Microbe-Associated Molecular Patterns (MAMPs) by cell surface receptors initiate a cascade of biochemical responses that include, among others, ion fluxes across the plasma membrane. A consequence of such event is a decrease in the concentration of extracellular H+ ions, which can be experimentally detected in plant cell suspensions as a shift in the pH of the medium. Thus, similarly to reactive oxygen species (ROS) accumulation, phosphorylation of MAP kinases and induction of defense-related genes, MAMP-induced medium alkalinization can be used as a proxy for the activation of plant immune responses. Here, we describe a detailed protocol for the measurement of medium alkalinization of tobacco BY-2 cell suspensions upon treatment with two different MAMPs: chitohexamers derived from fungal cell walls (NAG6; N-acetylglucosamine) and the flagellin epitope flg22, found in the bacterial flagellum. This method provides a reliable and fast platform to access MAMP-Triggered Immunity (MTI) in tobacco cell suspensions and can be easily adapted to other plant species as well as to other MAMPs. (AU)

FAPESP's process: 13/09878-9 - Functional characterization of a chitinase-like protein of Moniliophthora perniciosa with a potential role in fungal pathogenicity during the development of the witches' broom disease of cacao
Grantee:Gabriel Lorencini Fiorin
Support type: Scholarships abroad - Research Internship - Scientific Initiation
FAPESP's process: 09/50119-9 - Integrated and comparative study of three fungal diseases of cacao: witches' broom, frosty pod rot and brown-rot, aiming at understanding the pathogenic mechanisms for the development of control strategies
Grantee:Gonçalo Amarante Guimarães Pereira
Support type: Research Projects - Thematic Grants
FAPESP's process: 11/23315-1 - Functional characterization of a chitinase-like protein of Moniliophthora perniciosa with a potential role in fungal pathogenicity during the development of the witches' broom disease of cacao.
Grantee:Gabriel Lorencini Fiorin
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/06181-0 - Development of a CRISPR/Cas9-based methodology for genome engineering in the fungus Moniliophthora perniciosa, the causative agent of witches' broom disease of cacao
Grantee:Gabriel Lorencini Fiorin
Support type: Scholarships in Brazil - Master