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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Selective inhibition of Zophobas morio (Coleoptera: Tenebrionidae) luciferase-like enzyme luminescence by diclofenac and potential suitability for light-off biosensing

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Author(s):
Carvalho, Mariele C. [1] ; Tomazini, Atilio [2] ; Prado, Rogilene A. [3] ; Viviani, Vadim R. [1, 3]
Total Authors: 4
Affiliation:
[1] Fed Univ Sao Carlos UFSCar, Grad Program Evolut Genet & Mol Biol, Sao Carlos - Brazil
[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas, SP - Brazil
[3] Fed Univ Sao Carlos UFSCar, Grad Program Biotechnol & Environm Monitoring, Sorocaba - Brazil
Total Affiliations: 3
Document type: Journal article
Source: LUMINESCENCE; v. 36, n. 2 NOV 2020.
Web of Science Citations: 0
Abstract

The accumulation of toxic carboxylic compounds may cause severe effects on the environment and living organisms. A luciferase-like enzyme, previously cloned from the Malpighian tubules of the non-luminescent Zophobas morio mealworm, displays thioesterification activity with a wide range of carboxylic substrates, and produces weak red luminescence in the presence of ATP and firefly d-luciferin, a xenobiotic for this organism. To better investigate the function of this enzyme in carboxylic xenobiotic detoxification, we analyzed the inhibitory effect of different xenobiotic carboxylic acids on the luminescence activity of this enzyme, including environmental pollutants and pharmaceutical compounds. Noteworthy, the anti-inflammatory drug diclofenac severely inhibited this luciferase-like enzyme luminescence activity, both in in vitro (IC50 20 mu M) and in vivo in bacterial cells assays, when compared with other beetle luciferases. Similar results were obtained with its brighter I327S mutant. Kinetic analysis of diclofenac's effect on luminescence activity indicated mixed-type inhibition for both ATP and d-luciferin. Modelling studies showed five potential binding sites for diclofenac, including the coenzyme A binding site, which showed one of the highest binding constant. Taken together, these results raise the possibility of using this luciferase-like enzyme for the development of novel whole-cell luminescent biosensors for diclofenac and similar drugs. (AU)

FAPESP's process: 16/07312-6 - Structure and biological function of luciferase-like enzime of Arachnocampa luminosa lanterns (Diptera: Keroplatidae) and luminescent activity development by genetic engineering
Grantee:Mariele Cristina de Carvalho
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 14/18179-0 - Application of recombinant Zophobas morio luciferase-like enzyme for toxicity bioassays of xenobiotic carboxylic acids
Grantee:Mariele Cristina de Carvalho
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/05426-8 - Arthropod bioluminescence: biological diversity in Brazilian biomes, biochemical origin, structural/functional evolution of luciferases, molecular differentiation of lanterns, biotechnological, environmental and educational applications
Grantee:Vadim Viviani
Support type: Research Projects - Thematic Grants