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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In situ study of cellular immune response in human cutaneous lesions caused by Leishmania (Viannia) panamensis in Panama

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Author(s):
Gonzalez, Kadir [1, 2] ; Calzada, Jose Eduardo [1, 3] ; Tomokane, Thaise Yumie [2] ; Sandoval Pacheco, Carmen Maria [2] ; Araujo Flores, Gabriela Venicia [2] ; Castro Gomes, Claudia Maria [2] ; Pereira Corbett, Carlos Eduardo [2] ; Saldana, Azael [1, 4] ; Laurenti, Marcia Dalastra [2]
Total Authors: 9
Affiliation:
[1] Inst Conmemorat Gorgas Estudios Salud, Dept Parasitol Mol, Panama City - Panama
[2] Univ Sao Paulo, Fac Med, Dept Patol, Lab Patol Molestias Infecciosas, Sao Paulo - Brazil
[3] Univ Panama, Fac Med Vet, Panama City - Panama
[4] Univ Panama, Fac Med, Ctr Invest & Diagnost Enfermedades Parasitarias, Panama City - Panama
Total Affiliations: 4
Document type: Journal article
Source: PARASITE IMMUNOLOGY; v. 43, n. 3 OCT 2020.
Web of Science Citations: 0
Abstract

Aims: Leishmaniasis is considered a disease with multiple clinical/immunopathological characteristics, depending on the immunity of the host and the species of the parasite. In Panama, the most prevalent species that causes localized cutaneous leishmaniasis (LCL) is Leishmania (Viannia) panamensis, and its immune response is poorly studied. Therefore, we evaluated by immunohistochemistry, the in situ immune response during this infection. Methods and Results: Biopsies from Panamanian patients with LCL were collected and processed by histological techniques. Infection by L. (V.) panamensis was demonstrated by isolation in culture and molecular characterization by Hsp70-RFLP. The in situ immune response was assessed by immunohistochemistry. The immune response was characterized by predominance of T cells, mainly CD8 cells that showed positive correlation with IFN-gamma and Granzyme B. CD4 cells presented positive correlation with both IFN-gamma and IL-13, pointed by mixed cellular immune response. Regulatory response was characterized by FoxP3 cells, which showed positive correlation to IL-10 but not with TGF-beta. Conclusions: L. (V.) panamensis infection triggers a mixed cellular immune response, characterized by the presence of pro-inflammatory, anti-inflammatory and regulatory elements in the skin lesion of Panamanian patients. These data contribute to a better understanding of the immunopathogenesis of Leishmania Viannia infection in Panama. (AU)

FAPESP's process: 17/03141-5 - Characterization of histopathological changes and cellular immune response in cutaneous lesions of patients with Tegumentary Leishmaniasis in Panamá
Grantee:Kadir Amilcar Gonzalez Carrion
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 14/50315-0 - Leishmaniasis in Latin America: an advanced perspective on immunopathogenetic factors of cutaneous and visceral infection, immunomodulators of the sandflies vector saliva and immunogenic exo-antigens of Leishmania (L.) infantum chagasi as vaccine candidates
Grantee:Carlos Eduardo Pereira Corbett
Support type: Research Projects - Thematic Grants