Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

HPLC METHOD IMPROVEMENT FOR 4-NONYLPHENOL DETERMINATION IN ANAEROBIC REACTORS: DECREASING SOLVENT CONSUMPTION AND WASTE GENERATION

Full text
Author(s):
Henrique S. Dornelles [1] ; Fabrício Motteran [2] ; Isabel K. Sakamoto [3] ; Maria A. T. Adorno [4] ; Maria Bernadete A. Varesche [5]
Total Authors: 5
Affiliation:
[1] Universidade de São Paulo. Escola de Engenharia de São Carlos. Departamento de Engenharia Hidráulica e Saneamento - Brasil
[2] Universidade Federal de Pernambuco. Departamento de Engenharia Civil e Ambiental - Brasil
[3] Universidade de São Paulo. Escola de Engenharia de São Carlos. Departamento de Engenharia Hidráulica e Saneamento - Brasil
[4] Universidade de São Paulo. Escola de Engenharia de São Carlos. Departamento de Engenharia Hidráulica e Saneamento - Brasil
[5] Universidade de São Paulo. Escola de Engenharia de São Carlos. Departamento de Engenharia Hidráulica e Saneamento - Brasil
Total Affiliations: 5
Document type: Journal article
Source: Química Nova; v. 43, n. 8, p. 1048-1057, 2020-10-16.
Abstract

The aim of this study was to develop a chromatographic method for 4-NP determination in anaerobic batch reactors using low amount of organic solvents and also evaluate 4-NP degradation in the presence of different co-substrates. Chromatographic parameters was improved for stationary phase (C-8 column), mobile phase (acetonitrile/water solution 90:10, v:v), column temperature (40 ºC), eluent flow (0.8 mL min-1), injection volume (100 µL) and wavelengths in fluorescence detector (FLD) (λex=225 nm and λem=305 nm). Solid phase extraction (SPE) was performed for sample purification with smaller amount of solvents (methanol - 2 mL and ethanol - 1 mL) than previously reported. Reduction in reagent consumption was about 5.5 times for HPLC and about 19 times for SPE, according to previously reported. Method was validated according to the linearity (0.9951), selectivity and limits of detection and quantification were 15.7 µg L-1 and 55.6 µg L-1, respectively. The 4-NP removal in anaerobic batch reactors was higher than 50% (4-NP initial concentration of 398 µg L-1). Fumarate was a better co-substrate to favor methanogenesis in the presence of 4-NP, which provided higher organic matter removal, higher methane production speed and shorter start-up time for methane production. (AU)

FAPESP's process: 17/22850-7 - Microbial characterization of removal and degradation of 4-nonylphenol in fluidized bed biofilm reactor on increased scale
Grantee:Henrique de Souza Dornelles
Support type: Scholarships in Brazil - Master