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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae

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Author(s):
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Martinez-Bengochea, A. [1] ; Doretto, L. [1] ; Rosa, I. F. [1] ; Oliveira, M. A. [1] ; Silva, C. [1] ; Silva, D. M. Z. A. [1] ; Santos, G. R. [2, 3] ; Santos, J. S. F. [2, 3] ; Avelar, M. M. [2, 3] ; Silva, V, L. ; Lucianelli-Junior, Jr., D. [4] ; Souza, E. R. B. [5, 6] ; Silva, R. C. [5, 6] ; Stewart, A. B. [7] ; Nakaghi, L. S. O. [5, 6] ; Valentin, F. N. [4] ; Nobrega, R. H. [1]
Total Authors: 17
Affiliation:
[1] Sao Paulo State Univ, Inst Biosci, Dept Struct & Funct Biol, Reprod & Mol Biol Grp, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Fac Agr & Vet Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP - Brazil
[3] Sao Paulo State Univ, Aquaculture Ctr CAUNESP, Jaboticabal, SP - Brazil
[4] Univ Fed Para, UFPA, Fac Med, Lab Morfofisiol, Altamira, Para - Brazil
[5] Silva, L., V, Sao Paulo State Univ, Fac Agr & Vet Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP - Brazil
[6] Silva, L., V, Sao Paulo State Univ, Aquaculture Ctr CAUNESP, Jaboticabal, SP - Brazil
[7] West Virginia Univ, Dept Orthopaed Musculoskeletal Res, Morgantown, WV 26506 - USA
Total Affiliations: 7
Document type: Journal article
Source: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY; v. 248, OCT-NOV 2020.
Web of Science Citations: 0
Abstract

Gonadal sex differentiation in teleost fish shows greater plasticity as compared to other vertebrates, as it can be influenced by a variety of factors such as exogenous sex steroids. Exogenous estrogens, such as 17 beta-estradiol (E2), can induce feminization when administered during early embryonic development. However, the mechanisms underlying the E2-induced feminization are not fully understood, especially in Neotropical species. Therefore, the aim of this study was to evaluate the effects of E2 administration on the phenotypic sex characteristics, histological assessment of the gonads, and the expression of selected genes in Astyanax altiparanae exposed to dietary E2 prior to gonadal differentiation. M 4 days post-hatch (dph), groups of 30-40 undifferentiated larvae were fed with a diet containing varying amounts of E2 for 28 days, and fish were sampled at 90 dph. Previous studies revealed that ovary formation in A. altiparanae occurred at 58 dph, whereas the first sign of testis formation was found at 73 dph. In relation to the control, E2 exposure increased the proportion of phenotypic females in 120% and 148.4% for 4 and 6 mg E2/Kg, respectively. However, histological analysis revealed that treatments did not affect gonadal sex ratio between males and females, but induced intersex (testisova) in the group treated with 6 mg E2/Kg food. Treatment with E2 also altered gonadal transcript levels of a selected number of genes implicated in sexual differentiation. Males overexpressed dmrt1, sox9 and amh following E2 treatment as compared to control. Females showed increased mRNA levels of dmrtl and sox9, which might be related to the down-regulation of cyp19a1a after E2 exposure. In summary, E2 exposure during early gonadal development affected male secondary characteristics without changing the gonadal sex ratio, and altered expression of genes implicated in sexual differentiation. (AU)

FAPESP's process: 14/07620-7 - Spermatogonial stem cell bank to preserve the genotype of endangered and valuable teleost species
Grantee:Rafael Henrique Nóbrega
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 16/12101-4 - Selection, Culture and Transcriptome of spermatogonial stem cells of zebrafish, Danio rerio: a functional and genomics approach
Grantee:Lucas Benites Doretto
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 18/10265-5 - Endocrine and molecular mechanisms involved in the sexual differentiation of Medaka (Oryzias latipes) under conditions of thermal stress
Grantee:Ivana Felipe da Rosa
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/25313-4 - The role of AMH on sex differentiation and gametogenesis of teleost fish
Grantee:Marcos Antônio de Oliveira
Support Opportunities: Scholarships in Brazil - Doctorate