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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Revealing the Venomous Secrets of the Spider's Web

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Author(s):
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Esteves, Franciele Grego [1] ; Aparecido dos Santos-Pinto, Jose Roberto [1] ; Ferro, Milene [1] ; Sialana, Fernando J. [2] ; Smidak, Roman [2] ; Rares, Lucaciu Calin [3] ; Nussbaumer, Thomas [3] ; Rattei, Thomas [3] ; Bilban, Martin [4] ; Bacci Junior, Mauricio [1] ; Lubec, Gert [5] ; Palma, Mario Sergio [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo State UNESP, Inst Biosci Rio Claro, Dept Gen & Appl Biol, Ctr Study Social Insects, BR-13506900 Rio Claro - Brazil
[2] Univ Vienna, Dept Pharmaceut Chem, A-1090 Vienna - Austria
[3] Univ Vienna, Dept Microbiol & Ecosyst Sci, Div Computat Syst Biol, A-1090 Vienna - Austria
[4] Med Univ Vienna, Dept Lab Med & Core Facil Genom, A-1090 Vienna - Austria
[5] Paracelsus Med Univ, A-5020 Salzburg - Austria
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF PROTEOME RESEARCH; v. 19, n. 8, p. 3044-3059, AUG 7 2020.
Web of Science Citations: 0
Abstract

Orb-weaving spiders use a highly strong, sticky and elastic web to catch their prey. These web properties alone would be enough for the entrapment of prey; however, these spiders may be hiding venomous secrets in the web, which current research is revealing. Here, we provide strong proteotranscriptomic evidence for the presence of toxin/neurotoxin-like proteins, defensins, and proteolytic enzymes on the web silk from Nephila clavipes spider. The results from quantitative-based transcriptomic and proteomic approaches showed that silk-producing glands produce an extensive repertoire of toxin/neurotoxin-like proteins, similar to those already reported in spider venoms. Meanwhile, the insect toxicity results demonstrated that these toxic components can be lethal and/or paralytic chemical weapons used for prey capture on the web, and the presence of fatty acids in the web may be a responsible mechanism opening the way to the web toxins for accessing the interior of prey's body, as shown here. Comparative phylogenomic-level evolutionary analyses revealed orthologous genes among two spider groups, Araneomorphae and Mygalomorphae, and the findings showed protein sequences similar to toxins found in the taxa Scorpiones and Hymenoptera in addition to Araneae. Overall, these data represent a valuable resource to further investigate other spider web toxin systems and also suggest that N. clavipes web is not a passive mechanical trap for prey capture, but it exerts an active role in prey paralysis/killing using a series of neurotoxins. (AU)

FAPESP's process: 15/14220-8 - Proteometabolomic characterization of the spider web components Nephila clavipes used in prey capture strategy
Grantee:Franciele Grego Esteves
Support type: Scholarships in Brazil - Master
FAPESP's process: 17/10373-0 - Profiling the peptidomic and structural-functional characterization of lipid vesicles present in the Nephila clavipes web spider
Grantee:Franciele Grego Esteves
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/26451-9 - Bioprospecting and Structural Analysis of the Silk Proteins of Arthropods by a Proteomics Approach Using nanoLC-ESI-CID/ETD System
Grantee:José Roberto Aparecido dos Santos-Pinto
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/10430-0 - PROTEOTRANSCRIPTOMIC VIEW ON THE ROLE OF SPIDER SILK GLANDS DURING THE SILK SPINNING PROCESS
Grantee:José Roberto Aparecido dos Santos-Pinto
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 16/16212-5 - Natural proteopeptides from the Brazilian fauna, flora and microbiota as potential models for the rational development of new drugs of therapeutic use: isolation, structure elucidation, chemical synthesis and functional activity assays
Grantee:Mario Sergio Palma
Support type: BIOTA-FAPESP Program - Thematic Grants