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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A rationally identified marine GH1 beta-glucosidase has distinguishing functional features for simultaneous saccharification and fermentation

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Author(s):
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de Sousa, Amanda S. [1] ; de Melo, Ricardo R. [1] ; Miyamoto, Renan Y. [2, 1] ; Morais, Mariana A. B. [1] ; Andrade, Liliane P. [2, 1] ; Milan, Natalia [3, 1] ; de Avila, Mayara C. [3, 1] ; de Souza, Claudia M. [1] ; Adao, Regina C. [4, 5] ; Scarpassa, Josiane A. [1] ; Vieira, Plinio S. [1] ; dos Santos, V, Leandro ; Ramos, I, Carlos H. ; Murakami, Mario T. [6] ; Zanphorlin, Leticia M. [6]
Total Authors: 15
Affiliation:
[1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas - Brazil
[2] Univ Estadual Campinas, Inst Biol, Campinas - Brazil
[3] Univ Estadual Campinas, Fac Food Engn, Campinas - Brazil
[4] I, Univ Estadual Campinas, Inst Chem, Campinas, SP - Brazil
[5] NIH, Dynam Macromol Assembly Sect, Lab Cellular Imaging & Macromol Biophys, Bldg 10, Bethesda, MD 20892 - USA
[6] dos Santos, Leandro, V, Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas - Brazil
Total Affiliations: 6
Document type: Journal article
Source: BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR; AUG 2020.
Web of Science Citations: 0
Abstract

The classical route for second-generation ethanol from lignocellulosic biomass is hampered by high process costs, fostering the development of alternative strategies such as simultaneous saccharification and fermentation (SSF). However, the lack of compatible enzyme cocktails poses a challenge. In this study, the enzymeEmBgl from the marine bacteriumExiguobacterium marinumwas rationally identified based on structural and phylogenetic analyses, known desirable properties of close orthologs, and the ecological niche of its organism source.EmBgl is a multifunctional and glucose-tolerant enzyme that efficiently hydrolyzes cello-oligosaccharides due to a positive-subsite region that can accommodate long cello-oligosaccharides without imposing steric impediments. The efficacy ofEmBgl in an SSF process was demonstrated using pretreated sugarcane bagasse as feedstock, yielding 28 g L(-1)of ethanol in 30 h. The distinguishing functional properties ofEmBgl and its successful utilization in an SSF process highlight its potential in biotechnological applications in which lignocellulose deconstruction is desirable under milder temperatures (c) 2020 Society of Chemical Industry and John Wiley \& Sons, Ltd. (AU)

FAPESP's process: 16/19995-0 - Analysis of structural and functional diversity of GH43 enzymes from Xanthomonas axonopodis pv. citri: biological implications and potential biotechnological applications
Grantee:Mariana Abrahão Bueno de Morais
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/14253-9 - Evaluation of the biotechnological potential of the cellulolytic system of Xanthomonas axonopodis pv. citri: a structural, functional and applied approach
Grantee:Ricardo Rodrigues de Melo
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/02865-2 - Molecular investigation of new xylose isomerases for application to lignocellulosic materials fermentation
Grantee:Renan Yuji Miyamoto
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 15/26982-0 - Exploring novel strategies for depolymerization of plant cell-wall polysaccharides: from structure, function and rational design of glycosyl hydrolases to biological implications and potential biotechnological applications
Grantee:Mário Tyago Murakami
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/06509-0 - Understanding the enzymatic system involved in the degradation and utilization of xyloglucans from the plant pathogen Xanthomonas axonopodis pv. citri
Grantee:Plínio Salmazo Vieira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 19/08855-1 - New mechanisms of P450: an enzymatic strategy for renewable hydrocarbons
Grantee:Leticia Maria Zanphorlin
Support type: Regular Research Grants