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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions

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Zanchetta, Flavia Cristina [1] ; Trinca, Rafael Bergamo [2] ; Gomes Silva, Juliany Lino [1] ; Cunha Breder, Jessica da Silva [1] ; Cantarutti, Thiago Anselmo [1] ; Consonni, Silvio Roberto [3] ; Moraes, Angela Maria [2] ; de Araujo, Eliana Pereira [1] ; Abdalla Saad, Mario Jose [4] ; Adams, Gary G. [5] ; Melo Lima, Maria Helena [1]
Total Authors: 11
[1] Univ Estadual Campinas, Sch Nursing, BR-13083887 Campinas - Brazil
[2] Univ Estadual Campinas, Sch Chem Engn, Dept Engn Mat & Bioproc, BR-13083852 Campinas - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, BR-13083970 Campinas - Brazil
[4] Univ Estadual Campinas, Dept Internal Med, BR-13083887 Campinas - Brazil
[5] Univ Nottingham, Fac Med, Queens Med Ctr, Sch Hlth Sci, C Floor, South Block Link, Nottingham NG7 2HA - England
Total Affiliations: 5
Document type: Journal article
Source: POLYMERS; v. 12, n. 7 JUL 2020.
Web of Science Citations: 0

Polycaprolactone (PCL) is a synthetic polymer with good mechanical properties that are useful to produce biomaterials of clinical application. It can be successfully combined with chitosan, which enhances the biomaterial properties through the modulation of molecular and cellular mechanisms. The objective of this study was to evaluate the effects of the use of electrospun fibrous membranes consisting of polycaprolactone (PCL) or polycaprolactone coated with chitosan and poly(ethylene oxide) (PCL+CHI/PEO) on mouse skin lesions. Sixty four Black-57 mice were divided into PCL and PCL+CHI/PEO groups. A 1 cm(2)lesion was made on the animals' backs, and the membranes were sutured in place. The tissues were extracted on the 3rd, 7th, and 14th days after the lesion. The tissues were analyzed by histology with Hematoxylin and Eosin (H\&E) and Sirius Red stains, morphometry, immunohistochemistry, and Western blot. On the 3rd, 6th, and 9th days after the lesion, the PCL+CHI/PEO group showed a higher wound-healing rate (WHR). On the 3 day, the PCL+CHI/PEO group showed a greater amount of inflammatory infiltrate, greater expression of proliferating cell nuclear antigen (PCNA), and smooth muscle actin (alpha-SMA) (p< 0.05) compared to the PCL group. On the 7th day after the lesion, the PCL+CHI/PEO group showed a greater amount of inflammatory infiltrate, expression of Tumor Necrosis Factor (TNF-alpha) and PCNA (p< 0.05). In addition, it showed a greater immunolabeling of Monocyte Chemoattractant Protein-1 (MCP-1) and deposition of collagen fibers compared to the PCL group. The PCL+CHI/PEO membrane modulated the increase in the inflammatory infiltrate, the expression of MCP-1, PCNA, and alpha-SMA in lesions of mice. (AU)

FAPESP's process: 15/15134-8 - Effect topical insulin of the inflammataory and proliferetiva phase on 2nd degree-burns in diabetic-rats
Grantee:Maria Helena de Melo Lima
Support Opportunities: Regular Research Grants