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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fungistatic Action of N-Acetylcysteine onCandida albicansBiofilms and Its Interaction with Antifungal Agents

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Author(s):
Bezerra Santos Nunes, Thais Soares [1] ; Rosa, Leticia Matheus [1] ; Vega-Chacon, Yuliana [1] ; de Oliveira Mima, Ewerton Garcia [1]
Total Authors: 4
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Lab Appl Microbiol, BR-14801903 Araraquara, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: MICROORGANISMS; v. 8, n. 7 JUL 2020.
Web of Science Citations: 0
Abstract

Therapies targeted to fungal biofilms, mainly against the matrix, and therapies that do not induce microbial resistance are relevant. N-acetylcysteine (NAC), a mucolytic agent, has shown antimicrobial action. This study evaluated the effect of NAC against fluconazole-susceptible (CaS) and -resistant (CaR)Candida albicans. The susceptibility of planktonic cultures to NAC, the effect of NAC on biofilms and their matrix, the interaction of NAC with antifungal agents, and confocal microscopy were evaluated. Data were analyzed descriptively and by the ANOVA/Welch and Tukey/Gomes-Howell tests. The minimum inhibitory concentration (MIC) of NAC was 25 mg/mL for both strains. NAC significantly reduced the viability of both fungal strains. Concentrations higher than the MIC (100 and 50 mg/mL) reduced the viability and the biomass. NAC at 12.5 mg/mL increased the fungal viability. NAC also reduced the soluble components of the biofilm matrix, and showed synergism with caspofungin against planktonic cultures of CaS, but not against biofilms. Confocal images demonstrated that NAC reduced the biofilm thickness and the fluorescence intensity of most fluorochromes used. High concentrations of NAC had similar fungistatic effects against both strains, while a low concentration showed the opposite result. The antibiofilm action of NAC was due to its fungistatic action. (AU)

FAPESP's process: 18/02513-9 - Quantification of Candida albicans, effect of efflux pump inhibitors, N-acetylcystein and light-active micelles and validation of an in vivo model of denture stomatitis
Grantee:Ewerton Garcia de Oliveira Mima
Support Opportunities: Regular Research Grants