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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Feasibility of methylatedctDNAdetection in plasma samples of oropharyngeal squamous cell carcinoma patients

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de Jesus, Lais Machado [1] ; dos Reis, Mariana Bisarro [1] ; Carvalho, Raiany Santos [2] ; Scapulatempo Neto, Cristovam [1] ; de Almeida, Gisele Caravina [3] ; Laus, Ana Carolina [1] ; Marczynski, Gabriella Taques [1] ; Leal, Leticia Ferro [1] ; Melendez, Matias Eliseo [1, 4, 5] ; de Marchi, Pedro [6] ; Manuel Reis, Rui [7, 1, 8] ; Carvalho, Andre Lopes [1] ; de Carvalho, Ana Carolina [1]
Total Authors: 13
[1] Barretos Canc Hosp, Mol Oncol Res Ctr, Rua Antenor Duarte Vilela 1331, BR-14784400 Barretos, SP - Brazil
[2] Barretos Canc Hosp, Res Support Ctr, Barretos - Brazil
[3] Barretos Canc Hosp, Dept Pathol, Barretos - Brazil
[4] Pele Little Prince Res Inst, Curitiba, Parana - Brazil
[5] Little Prince Complex, Curitiba, Parana - Brazil
[6] Barretos Canc Hosp, Dept Med Oncol, Barretos - Brazil
[7] ICVS 3Bs PT Govt Associate Lab, Braga - Portugal
[8] Univ Minho, Life & Hlth Sci Res Inst ICVS, Med Sch, Braga - Portugal
Total Affiliations: 8
Document type: Journal article
Web of Science Citations: 0

Background Oropharyngeal squamous cell carcinomas (OpSCCs) are commonly associated with high rates of treatment failure. Objectives To evaluate methylation-based markers in plasma from OpSCC patients as emerging tools for accurate/noninvasive follow-up. Methods Pretreatment formalin-fixed paraffin-embedded (FFPE) biopsies (n = 52) and paired plasma (n = 15) were tested for the methylation ofCCNA1,DAPK,CDH8, andTIMP3by droplet digital PCR (ddPCR). Results Seventy-one percent (37/52) of the biopsies showed methylation of at least one of the evaluated genes and tumorCCNA1methylation was associated with recurrence-free survival. Methylated circulating tumor DNA (meth-ctDNA) was detected in 11/15 (73.3%) plasma samples; conversely, plasma samples from healthy controls were all negative for DNA methylation (area under the curve = 0.867; 95% confidence interval = 0.720-1.000). Additionally, preliminary results on the detection of meth-ctDNA in plasma collected during follow-up closely matched patient outcome. Conclusions The results suggest the feasibility of detecting meth-ctDNA in plasma using ddPCR and a possible application on routine setting after further validation. (AU)

FAPESP's process: 13/13834-7 - Comparison of the methylation profile of specific genes between HPV-positive and HPV-negative tumors from patients with head and neck squamous cell carcinoma
Grantee:Ana Carolina de Carvalho Peters
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/22658-9 - Detection and characterization of molecular alterations in tissue samples and body fluids from patients with head and neck tumors
Grantee:Lais Machado de Jesus
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 15/01286-0 - Characterization of genetic and epigenetic alterations in specific genes in HPV-positive and HPV-negative tumors of patients with head and neck squamous cell carcinoma
Grantee:André Lopes Carvalho
Support Opportunities: Regular Research Grants