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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Aqueous humor renin, angiotensin I, and angiotensin II activity in primary open-angle glaucoma

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Author(s):
Valéria Batista Boreck Seki [1] ; Guilherme Rabelo de Souza [2] ; Andre Messias [3] ; Dulce Elena Casarini [4] ; Jayter Silva de Paula [5]
Total Authors: 5
Affiliation:
[1] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Department of Ophthalmology - Brasil
[2] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Department of Ophthalmology - Brasil
[3] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Department of Ophthalmology - Brasil
[4] Universidade Federal de São Paulo. Department of Medicine. Nephrology Division - Brasil
[5] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Department of Ophthalmology - Brasil
Total Affiliations: 5
Document type: Journal article
Source: Arquivos Brasileiros de Oftalmologia; v. 83, n. 4, p. 318-322, 2020-07-29.
Abstract

ABSTRACT Purpose: The renin-angiotensin system is involved in the pathogenesis of retinal ischemic conditions and glaucoma. Our objective was to evaluate the renin, angiotensinconverting enzyme 1, and angiotensin-converting enzyme 2 activities in aqueous humor and blood samples of patients with and without primary open-angle glaucoma. Methods: We analyzed samples from 56 participants who underwent ocular surgeries. The patients were divided into two groups: patients with cataract alone (n=28) and patients with cataract and primary open-angle glaucoma (n=28). Venous blood (2 ml) and aqueous humor (150 µl, via paracentesis) samples were collected during phacoemulsification (cataract only) or glaucoma surgery (cataract and primary open-angle glaucoma). The serum and aqueous humor renin, angiotensin-converting enzyme 1, and angiotensin-converting enzyme 2 activities of all patients were evaluated by fluorimetric assays, and results were analyzed by using multivariate regression analysis. Results: Both the aqueous humor renin activity and renin activity aqueous humor/serum ratio were significantly lower in patients with cataract and primary open-angle glaucoma than in patients with cataract only [(mean ± SE): 0.018 ± 0.006 ng/ml/h vs 0.045 ± 0.009 ng/ml/h, p<0.001; 0.05 ± 0.02 vs 0.13 ± 0.05, p=0.025]. Multivariate analyses showed a significant relationship between lower aqueous humor renin activity and primary open-angle glaucoma [coefficient (±SE): -0.029 ± 0.013, p=0.026]. Conclusions: Our results showed that patients with primary open-angle glaucoma had lower aqueous humor renin activity. As timolol eye drops were used by most of the primary open-angle glaucoma patients, we propose that a large sample of washed-out patients should be studied in the future to discriminate the involvement of b-blocker treatment in the aqueous humor renin activity. (AU)

FAPESP's process: 16/09515-1 - The role of renin and (pro)renin receptors in pathologic processes related to the aqueous humor outflow
Grantee:Jayter Silva de Paula
Support Opportunities: Regular Research Grants