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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Triple quadrupole-mass spectrometry protocols for the analysis of NBOMes and NBOHs in blotter papers

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de Morais, Damila Rodrigues [1] ; da Cunha, Kelly Francisco [2] ; Rodrigues, Tais Betoni [2] ; Lanaro, Rafael [2, 3] ; Barbosa, Lilian de Melo [4] ; Zacca, Jorge Jardim [5] ; Eberlin, Marcos Nogueira [1] ; Costa, Jose Luiz [6, 3]
Total Authors: 8
[1] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Fac Med Sci, Dept Pharmacol, BR-13083887 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Fac Med Sci, Campinas Poison Control Ctr, BR-13083970 Campinas, SP - Brazil
[4] Criminalist Inst, Tech Sci Police Superintendence, BR-13018170 Campinas, SP - Brazil
[5] Brazilian Fed Police, Sci & Tech Dept PF, BR-70037900 Brasilia, DF - Brazil
[6] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083871 Campinas, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Forensic Science International; v. 309, APR 2020.
Web of Science Citations: 0

NBOMe and NBOH are new psychoactive substances with potent activity on serotonin 5-HT2a receptors causing serious toxic effects, including serotonin toxidrome and death. The aim of this work was to develop a comprehensive MS/MS protocol, using triple quadrupole mass spectrometers coupled to LC and GC, for rapid screening and quantitation of NBOMes and NBOHs in seized blotter papers. Different scan methods (neutral loss, precursor ion or multiple reaction monitoring) were used to obtain structural information of phenylethylamine class. The developed protocol was validated for qualitative and quantitative analysis, showing a satisfactory limit of detection (1 ng/mL), with excellent selectivity, imprecision (intra and interday imprecision lower than 1.2 % RSD) and accuracy (between -7.1 and +5.6 %, n = 15), as well as bias values. The analysis of real samples shown that NBOH compounds were the most frequently detected, with concentrations ranging from 0.1 to 1,929 mu g per blotter sample. Triple quadrupole mass spectrometers can be a useful tool for identification of new psychoactive substances. A comprehensive protocol using both LC-MS/MS and GC-MS/MS, with different scanning modes, have been developed and showed to be useful to screening NBOMe and NBOH in blotter papers. (C) 2020 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 18/00432-1 - The Toxicology of New Psychoactive Substances (NSP): epidemiology of consumption by the analysis of hair and oral fluid samples
Grantee:José Luiz da Costa
Support type: Regular Research Grants
FAPESP's process: 16/23157-0 - Development of a protocol for new synthetic cannabinoids and NBOMe (2-(2,5-dimethoxy)-N-(2-methoxybenzyl)ethan-1-amine) derivatives) analysis in scan mode precursor ion and neutral loss by GC-MS / MS and LC-MS / MS
Grantee:Damila Rodrigues de Morais
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/11849-0 - The toxicology of New Psychoactive Substances (NSP): epidemiology of consumption by oral fluid samples and characterization of their in vitro metabolism
Grantee:Kelly Francisco da Cunha
Support type: Scholarships in Brazil - Doctorate