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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Solventless separation of underivatized biogenic amines by sequential injection chromatography

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Gama, Mariana R. [1, 2] ; Rocha, Fabio R. P. [1]
Total Authors: 2
[1] Univ Sao Paulo, Ctr Nucl Energy Agr, Ave Centenario 303, BR-13400970 Piracicaba, SP - Brazil
[2] Univ Fed Rio Grande do Sul, Ave Bento Goncalves 9500, BR-91540000 Porto Alegre, RS - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Microchemical Journal; v. 156, JUL 2020.
Web of Science Citations: 1

Biogenic amines (BA) are markers of food freshness and indicators of inadequate food processing and storage conditions. Because BA determination is important for food quality evaluation, and considering the relatively complex analytical procedures available, new approaches are required for their determination. In this work, an environmentally friendly analytical procedure is proposed for BA determination in cheese, involving separation by sequential injection chromatography without organic solvents or chemical derivatization of the analytes. Sample preparation step was carried out by acid protein precipitation and low-temperature partition with a reduced volume of organic solvent (500 mu L of acetonitrile). Because of the previously mentioned characteristics, an analytical eco-scale = 91 was achieved. Histamine, tyramine, and tryptamine were separated in a cyanopropyl monolithic column in the isocratic elution mode, with 3.0 mmol L-1 phosphoric acid aqueous solution as mobile phase. The analytes were quantified from their intrinsic absorption at 210 nm. The analysis time was less than 3 min, with good chromatographic efficiency (N >= 75000 plates m(-1)). For the three analytes, LOD within 0.05-0.10 mg kg(-1), LOQ within 0.14-0.30 mg kg(-1), and linear response ranges up to 10 mg kg(-1) (histamine and tyramine) and 15 mg kg(-1) (tryptamine) were achieved with good precision (CV = 3.0%, n = 12). The proposed procedure was successfully applied to the determination of tyramine in cheese samples, with recoveries in the 81-128% range. (AU)

FAPESP's process: 17/08611-0 - Advances in sequential injection chromatography: two-dimensional chromatography and development of new stationary phases
Grantee:Mariana Roberto Gama Sato
Support Opportunities: Scholarships in Brazil - Post-Doctoral