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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Generation of induced pluripotent stem cells from large domestic animals

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Bressan, Fabiana Fernandes [1, 2, 3] ; Bassanezze, Vinicius [4, 5] ; de Figueiredo Pessoa, Lais Vicari [1, 2, 3] ; Sacramento, Chester Bittencourt [5, 6] ; Malta, Tathiane Maistro [7, 3] ; Kashima, Simone [3] ; Fantinato Neto, Paulo [1, 2] ; Strefezzi, Ricardo De Francisco [1] ; Godoy Pieri, Naira Caroline [1] ; Krieger, Jose Eduardo [5] ; Covas, Dimas Tadeu [3] ; Meirelles, Flavio Vieira [1, 2, 3]
Total Authors: 12
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga - Brazil
[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Postgrad Program Anat Domest & Wild Anim, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Ctr Cell Based Therapy, Reg Blood Ctr, Ribeirao Preto - Brazil
[4] Harvard Med Sch, Brigham & Womens Hosp, Boston, MA 02115 - USA
[5] Univ Sao Paulo, Heart Inst InCor, Fac Med, Sao Paulo - Brazil
[6] Cornell Univ, Weill Cornell Med, Ithaca, NY - USA
[7] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Source: STEM CELL RESEARCH & THERAPY; v. 11, n. 1 JUN 25 2020.
Web of Science Citations: 0

Background: Induced pluripotent stem cells (iPSCs) have enormous potential in developmental biology studies and in cellular therapies. Although extensively studied and characterized in human and murine models, iPSCs from animals other than mice lack reproducible results. Methods: Herein, we describe the generation of robust iPSCs from equine and bovine cells through lentiviral transduction of murine or human transcription factors Oct4, Sox2, Klf4, and c-Myc and from human and murine cells using similar protocols, even when different supplementations were used. The iPSCs were analyzed regarding morphology, gene and protein expression of pluripotency factors, alkaline phosphatase detection, and spontaneous and induced differentiation. Results: Although embryonic-derived stem cells are yet not well characterized in domestic animals, generation of iPS cells from these species is possible through similar protocols used for mouse or human cells, enabling the use of pluripotent cells from large animals for basic or applied purposes. Herein, we also infer that bovine iPS (biPSCs) exhibit similarity to mouse iPSCs (miPSCs), whereas equine iPSs (eiPSCs) to human (hiPSCs). Conclusions: The generation of reproducible protocols in different animal species will provide an informative tool for producing in vitro autologous pluripotent cells from domestic animals. These cells will create new opportunities in animal breeding through transgenic technology and will support a new era of translational medicine with large animal models. (AU)

FAPESP's process: 15/26818-5 - Investigation of cellular and molecular mechanisms on in vitro induced toti- and pluripotency acquisition - a translational approach
Grantee:Fabiana Fernandes Bressan
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 11/08376-4 - Effects of genetic induction and reprogramming through nuclear transfer in in vitro bovine embryo production
Grantee:Flávio Vieira Meirelles
Support Opportunities: Regular Research Grants
Grantee:Fabiana Fernandes Bressan
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/13686-8 - Induced reprogramming of bovine somatic cells into induced pluripotent cells (iPS) and primordial germ cells-like (PGCLs)
Grantee:Fabiana Fernandes Bressan
Support Opportunities: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC