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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Novel nanocarrier of miconazole based on chitosan-coated iron oxide nanoparticles as a nanotherapy to fight Candida biofilms

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Arias, Lais Salomao [1] ; Pessan, Juliano Pelim [1] ; de Souza Neto, Francisco Nunes [1] ; Ramos Lima, Bruno Henrique [2] ; de Camargo, Emerson Rodrigues [3] ; Ramage, Gordon [4] ; Botazzo Delbem, Alberto Carlos [1] ; Monteiro, Douglas Roberto [1, 5]
Total Authors: 8
[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Prevent & Restorat Dent, BR-16015050 Aracatuba, SP - Brazil
[2] nChemi Engn Mat, BR-13560460 Sao Carlos, SP - Brazil
[3] Fed Univ Sao Carlos UFSCar, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil
[4] Univ Glasgow, Coll Med Vet & Life Sci, Glasgow Dent Sch, Sch Med Dent & Nursing, Oral Sci Res Grp, Glasgow G2 3JZ, Lanark - Scotland
[5] Univ Western Sao Paulo UNOESTE, Grad Program Dent GPD Masters Degree, BR-19050920 Presidente Prudente, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Web of Science Citations: 0

Overexposure of microorganisms to conventional drugs has led to resistant species that require new treatment strategies. This study prepared and characterized a nanocarrier of miconazole (MCZ) based on iron oxide nanoparticles (IONPs) functionalized with chitosan (CS), and tested its antifungal activity against biofilms of Candida albicans and Candida glabrata. IONPs-CS-MCZ nanocarrier was prepared by loading MCZ on CS-covered IONPs and characterized by physicochemical methods. Minimum inhibitory concentration (MIC) of the nanocarrier was determined by the microdilution method. Biofilms were developed (48 h) in microtiter plates and treated with MCZ-carrying nanocarrier at 31.2 and 78 mu g/mL, in both the presence and absence of an external magnetic field (EMF). Biofilms were evaluated by total biomass, metabolic activity, cultivable cells (CFU), extracellular matrix components, scanning electron microscopy and confocal microscopy. Data were analyzed by two-way ANOVA and Holm-Sidak test (p < 0.05). A nanocarrier with diameter lower than 50 nm was obtained, presenting MIC values lower than those found for MCZ, and showing synergism for C. albicans and indifference for C. glabrata (fractional inhibitory concentration indexes of < 0.12 and < 0.53, respectively). IONPs-CS-MCZ did not affect total biomass and extracellular matrix. IONPs-CS-MCZ containing 78 mu g/mL MCZ showed a superior antibiofilm effect to MCZ in reducing CFU and metabolism for single biofilms of C. albicans and dual-species biofilms. The EMF did not improve the nanocarrier effects. Microscopy confirmed the antibiofilm effect of the nanocarrier. In conclusion, IONPs-CS-MCZ was more effective than MCZ mainly against C. albicans planktonic cells and number of CFU and metabolism of the biofilms. (AU)

FAPESP's process: 17/24416-2 - Synthesis and antibiofilm effect of two new carrier nanosystems of antifungal drugs
Grantee:Douglas Roberto Monteiro
Support Opportunities: Regular Research Grants