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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Rainbow trout slow myoblast cell culture as a model to study slow skeletal muscle, and the characterization of mir-133 and mir-499 families as a case study

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Author(s):
da Silva Duran, Bruno Oliveira [1] ; Dal-Pai-Silva, Maeli [1, 2] ; Garcia de la Serrana, Daniel [3, 4]
Total Authors: 3
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Morphol, BR-18618689 Botucatu, SP - Brazil
[2] Garcia de la Serrana, Daniel, Univ Barcelona, Fac Biol, Dept Cell Biol Physiol \& Immunol, Barcelona 08028, Spain.da Silva Duran, Bruno Oliveira, Sao Paulo State Univ UNESP, Inst Biosci, Dept Morphol, BR-18618689 Botucatu, SP - Brazil
[3] Univ St Andrews, Sch Biol, Scottish Oceans Inst, St Andrews KY16 8LB, Fife - Scotland
[4] Univ Barcelona, Fac Biol, Dept Cell Biol Physiol & Immunol, Barcelona 08028 - Spain
Total Affiliations: 4
Document type: Journal article
Source: Journal of Experimental Biology; v. 223, n. 2 JAN 2020.
Web of Science Citations: 3
Abstract

Muscle fibres are classified as fast, intermediate and slow. In vitro myoblast cell culture model from fast muscle is a very useful tool to study muscle growth and development; however, similar models for slow muscle do not exist. Owing to the compartmentalization of fish muscle fibres, we have developed a slow myoblast cell culture for rainbow trout (Oncorhynchus mykiss). Slow and fast muscle-derived myoblasts have similar morphology, but with differential expression of slow muscle markers such as slow myhc, sox6 and pgc-1a. We also characterized the mir-133 and mir-499 microRNA families in trout slow and fast myoblasts as a case study during myogenesis and in response to electrostimulation. Three mir-133 (a-1a, a-1b and a-2) and four mir-499 (aa, ab, ba and bb) paralogues were identified for rainbow trout and named base on their phylogenetic relationship to zebrafish and Atlantic salmon orthologues. Omy-mir-499ab and omy-mir-499bb had 0.6 and 0.5-fold higher expression in slow myoblasts compared with fast myoblasts, whereas mir-133 duplicates had similar levels in both phenotypes and little variation during development. Slow myoblasts also showed increased expression for omy-mir-499b paralogues in response to chronic electrostimulation (7-fold increase for omy-mir-499ba and 2.5-fold increase for omy-mir-499bb). The higher expression of mir-499 paralogues in slow myoblasts suggests a role in phenotype determination, while the lack of significant differences of mir-133 copies during culture development might indicate a different role in fish compared with mammals. We have also found signs of sub-functionalization of mir-499 paralogues after electrostimulation, with omy-mir-499b copies more responsive to electrical signals. (AU)

FAPESP's process: 15/03234-8 - The pacu (Piaractus mesopotamicus) myoblast cell culture as a model to understand muscle growth regulation in Ostariophysi superorder
Grantee:Bruno Oliveira da Silva Duran
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/05009-4 - Comparative and integrative analysis of the transcriptome and the microRNAoma in skeletal muscle of Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu) and its hybrid, tambacu: molecular, in silico, in vitro and in vivo approaches.
Grantee:Maeli Dal Pai
Support type: Regular Research Grants
FAPESP's process: 16/19683-9 - The slow myoblast cell culture as a model to understand the myogenesis and growth of slow-twitch muscle
Grantee:Bruno Oliveira da Silva Duran
Support type: Scholarships abroad - Research Internship - Doctorate