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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

microRNA-138-5p as a Worse Prognosis Biomarker in Pediatric, Adolescent, and Young Adult Osteosarcoma

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Roberto, Gabriela Molinari [1] ; Lira, Regia Caroline [2] ; Delsin, Lara Elis [3] ; Vieira, Gabriela Maciel [3] ; Silva, Marcela Oliveira [4] ; Hakime, Rodrigo Guedes [5] ; Yamashita, Mauricio Eiji [6] ; Engel, Edgard Eduard [6] ; Scrideli, Carlos Alberto [4] ; Tone, Luiz Gonzaga [4] ; Brassesco, Maria Sol [5, 7]
Total Authors: 11
[1] Univ Sao Paulo, Reg Blood Ctr, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Pediat, Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Genet, Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Cell Biol, Sao Paulo - Brazil
[5] Univ Sao Paulo, Dept Biomech Med & Rehabil Locomotor Syst, Ribeirao Preto Med Sch, Sao Paulo - Brazil
[6] Univ Sao Paulo, Fac Philosophy Sci & Letter Ribeirao Preto, Dept Biol, Sao Paulo - Brazil
[7] FFCLRP USP, Dept Biol, Av Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Source: Pathology & Oncology Research; v. 26, n. 2, p. 877-883, APR 2020.
Web of Science Citations: 4

Osteosarcoma (OS) is the most common primary malignant bone tumor with two peaks of incidence, in early adolescence and the elderly. Patients affected with this malignancy often present metastatic disease at diagnosis, and despite multimodality therapy, survival has not improved substantially over the past 3 decades. Recently, miR-138-5p, proposed as a crucial intracellular mediator of invasion, has been recognized to target the Rho-associated coiled-coil containing protein kinase 2 (ROCK2). Dysregulation of ROCK1 and ROCK2 was also described in OS, being associated to higher metastasis incidence and worse prognosis. Nonetheless, the specific roles of miR-138-5p in pediatric and young adult OS and its ability to modulate these kinases remain to be established. Thus, in the present study, the expression levels miR-138-5p were evaluated in a consecutive cohort of exclusively pediatric and young adult primary OS samples. In contrast to previous reports that included adult tissues, our results showed upregulation of miR-138-5p associated with reduced event-free survival and relapsed cases. In parallel, ROCK1 mRNA levels were significantly reduced in tumor samples and negatively correlated with miR-138-5p. Similar correlations were observed after studying the profiles of ROCK1 and ROCK2 by immunohistochemistry. Our data present miR-138-5p as a consistent prognostic factor in pediatric and young adult OS, reinforcing its participation in the post-transcriptional regulation of ROCK kinases. (AU)

FAPESP's process: 14/03877-3 - Evaluation of rock kinases and their interaction with microRNAs in bone sarcomas of childhood: implications for tumor progression and invasion
Grantee:María Sol Brassesco Annichini
Support Opportunities: Regular Research Grants