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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Endothelium modulates electrical field stimulation-induced contractions of Chelonoidis carbonaria aortic rings

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Author(s):
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Campos, Rafael [1, 2] ; Jacintho, Felipe Fernandes [1] ; Britto-Junior, Jose [1] ; Monica, Fabiola Z. [1] ; Oliveira Justo, Alberto Fernando [1] ; Pupo, Andre Sampaio [3] ; Moreno, Ronilson Agnaldo [1] ; de Souza, Valeria Barbosa [1] ; Schenka, Andre Almeida [1] ; Antunes, Edson [1] ; De Nucci, Gilberto [4, 1]
Total Authors: 11
Affiliation:
[1] Univ Estadual Campinas, Fac Med Sci, Dept Pharmacol, UNICAMP, Campinas - Brazil
[2] Ceara State Univ UECE, Super Inst Biomed Sci, BR-60714903 Fortaleza, Ceara - Brazil
[3] UNESP Sao Paulo State Univ, Inst Biosci Botucatu, Dept Pharmacol, Botucatu, SP - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 233, JUL 2020.
Web of Science Citations: 0
Abstract

The role of endothelium in the electrical-field stimulation (EFS)-induced contractions of Chelonoidis carbonaria aorta was investigated. Contractions were evaluated in the presence and absence of L-NAME (100 mu M), tetrodotoxin (1 mu M), phentolamine (10 and 100 mu M), phenoxybenzamine (1 and 10 mu M), prazosin (100 mu M), idazoxan (100 mu M), atropine (10 mu M), D-tubocurarine (10 mu M) or indomethacin (10 mu M). EFS-induced contraction was also carried out in endothelium-denuded rings. EFS-induced contraction was investigated by the sandwich assay. Concentration curves to endothelin-1 (0.1-100 nM) and U46619 (0.001-100 mu M) were also constructed to calculate both Emax and EC50. EFS at 16 Hz contracted Chelonoidis aorta, which was almost abolished by the endothelium removal. The addition of L-NAME increased the EFS response (2.0 +/- 0.4 and 8.3 +/- 1.9 mN). In L-NAME treated aortic rings, tetrodotoxin did not change the EFS-response (5.1 +/- 1.8 and 4.9 +/- 1.7 mN). Indomethacin, atropine and d-tubucurarine also did not affect the EFS-response. Phentolamine at 10 mu M did not change the EFS-induced contraction; however, at 100 mu M, reduced it (3.9 +/- 1 and 1.9 +/- 0.3 mN). Prazosin and idazoxan did not change EFS-induced contractions. Phenoxybenzamine at 1 reduced by 76% (9.6 +/- 3.4 and 2.3 +/- 0.8 mN) and at 10 mu M by 90% the EFS response. Immunohistochemistry identified tyrosine hydroxylase in the endothelium and brain, whereas S100 protein was found only in brain. In conclusion, endothelium modulates EFS-induced contractions in Chelonoidis aortic rings and this modulation may be due to endothelium-derived catecholamines, possibly dopamine. (AU)

FAPESP's process: 18/24971-9 - Physiological relevance of catecholamines produced by the endothelium
Grantee:Felipe Fernandes Jacintho
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 16/04731-8 - Assessment of potential anti-cancer stem cell activity of simvastatin, Copaifera oil-resin and novel nanoparticle s (graphene oxide modified with nickel and iron, and magnetite) in single or combined use, in human breast cancer derived cell lines
Grantee:André Almeida Schenka
Support type: Regular Research Grants
FAPESP's process: 16/09539-8 - Pharmacological, eletrophysiological and morphological characterization of a novel tetrodotoxin-resistant sodium channel coupled to corpus cavernosum of snakes
Grantee:Alberto Fernando Oliveira Justo
Support type: Scholarships in Brazil - Doctorate (Direct)