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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Aluminum, at an environmental concentration, associated with acidic pH and high water temperature, causes impairment of sperm quality in the freshwater teleost Astyanax altiparanae (Teleostei: Characidae)

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Author(s):
Silva Pinheiro, Joao Paulo [1] ; de Assis, Cecilia Bertacini [1] ; Sanches, Eduardo Antonio [2] ; Moreira, Renata Guimaraes [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Lab Metab & Reprod Aquat Organisms LAMEROA, Inst Biosci, Dept Physiol, Matao St, 14 Lane, 101 Room 220, Cidade Univ, Sao Paulo, SP - Brazil
[2] Univ Estadual Paulista, Fishery Engn Course, Nelson Brihi Badur Ave 430, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Environmental Pollution; v. 262, JUL 2020.
Web of Science Citations: 0
Abstract

Given the toxicity of metals, including aluminum (Al), and the effects of water temperature on ectotherms, we investigated the individual or association effect of these variables (Al + acidic pH + temperature changes) on sperm quality of Astyanax altiparanae. Mature males were divided into nine experimental groups based on the combination of each of three water temperatures (20, 25, and 30 degrees C) with neutral and acidic pH values (7.0 and 5.5, respectively) with or without 0.5 mg L-1 Al. The fish were subjected to subacute, semi-static exposure and at 24 and 96 h were evaluated for seminal parameters: (1) pH; (2) osmolality; (3) sperm concentration; (4) sperm morphology; (5) sperm kinetics; and (6) sperm ultrastructure. At 30 degrees C, Al caused a reduction in osmolality (24 and 96 h) and sperm concentration (24 h). When analysing sperm kinetics (30 s post-activation), Al caused a reduction in total motility at all temperatures (24 h), and when this exposure time was longer (96 h), both acidic pH and Al addition to the water caused sperm motility reduction. By analysing curvilinear velocity (VCL) 30 s after sperm activation (24 and 96 h), the acidic pH caused a reduction in sperm movement at 20 and 30 degrees C, but at 25 degrees C Al triggered this reduction. Finally, Al in the water caused ultrastructural changes in the sperm head, midpiece, and flagella regardless of water temperature. Also, it was found that the combination of Al at 30 degrees C caused a reduction in sperm head area while at 20 degrees C, Al triggered a reduction in the midpiece area. Therefore, acidity influenced some A. altiparanae sperm parameters but Al in the water accentuated these effects on seminal quality, especially seminal osmolality and sperm concentration, kinetics, and ultrastructure. This toxicity was also influenced by changes in water temperature. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 14/16320-7 - Impacts of climate/environmental change on the fauna: an integrative approach
Grantee:Carlos Arturo Navas Iannini
Support type: Research Program on Global Climate Change - Thematic Grants
FAPESP's process: 16/08770-8 - Evaluation of the semen quality, mutagenicity and genotoxicity in Astyanax altiparanae (Teleostei, Characidae) exposed to aluminum in different water temperatures
Grantee:João Paulo Silva Pinheiro
Support type: Scholarships in Brazil - Doctorate