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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular characterization of different preproGnRHs in Astyanax altiparanae (Characiformes): Effects of GnRH on female reproduction

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Author(s):
Chehade, Chayrra [1] ; Amaral, Fernanda G. [2, 3] ; Branco, Giovana S. [1] ; Cassel, Monica [4] ; De Jesus, Lazaro W. O. [5] ; Costa, Fabiano G. [6] ; Bordin, Silvana A. [3] ; Moreira, Renata G. [7] ; Borella, I, Maria
Total Authors: 9
Affiliation:
[1] I, Univ Sao Paulo, Fish Endocrinol Lab, Dept Cell & Dev Biol, Biomed Sci Inst, Av Prof Lineu Prestes 1524, Lab 426, BR-05508000 Sao Paulo, SP - Brazil
[2] Fed Univ Sao Paulo UNIFESP, Dept Physiol, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Dept Physiol & Biophys, Biomed Sci Inst, Sao Paulo - Brazil
[4] Mato Grosso Fed Inst Educ Sci & Technol IFMT, Dept Educ, Anim Sci, Alta Floresta Campus, Alta Floresta, MG - Brazil
[5] Fed Univ Alagoas UFAL, Lab Appl Anim Morphophysiol, Dept Histol & Embryol, Inst Biol Sci & Hlth, Maceio, Alagoas - Brazil
[6] State Univ Northern Parana UENP, Dept Biol Sci, Jacarezinho, Parana - Brazil
[7] Univ Sao Paulo, Lab Metab & Reprod Aquat Organisms, Dept Physiol, Inst Biosci, Sao Paulo, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Source: Molecular Reproduction and Development; v. 87, n. 6 MAY 2020.
Web of Science Citations: 0
Abstract

Gonadotropin-releasing hormone (GnRH) is a key molecule in the initiation of the hypothalamic-pituitary-gonadal axis. Thus, knowledge about GnRH may contribute to the effectiveness of species reproduction. Using a Neotropical tetra Astyanax altiparanae as a fish model species, the GnRH forms were characterized at the molecular level and the role of injected GnRHs in vivo was evaluated. The full-length complementary DNA (cDNA) sequences of preproGnRH2 (612 bp) and preproGnRH3 (407 bp) of A. altiparanae were obtained, and the GnRH1 form was not detected. The cDNA sequences of preproGnRH2 and preproGnRH3 were found to be conserved, but a change in the amino acid at position 8 of the GnRH3 decapeptide of A. altiparanae was observed. All the injected GnRHs stimulated lh beta messenger RNA (mRNA) expression but not fsh beta mRNA expression, and only GnRH2 was able to increase maturation-inducing steroid (MIS) levels and possibly stimulate oocyte release. Furthermore, only GnRH2 was able to start the entire reproductive hormonal cascade and induce spawning. (AU)

FAPESP's process: 10/17586-0 - Molecular characterization of GnRH of the Astyanax altiparanae (Garutti and Britski, 2000), its effect in vivo, and its temporal expression during the reproductive stimulus.
Grantee:Chayrra Chehade Gomes
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 11/09567-8 - Molecular cloning of GnRH of Astyanax altiparanae and its effect in vivo
Grantee:Maria Ines Borella
Support Opportunities: Regular Research Grants