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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Expression profiles of growth-related genes in two Nile tilapia strains and their crossbred provide insights into introgressive breeding effects

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Author(s):
Herkenhoff, M. E. [1] ; Ribeiro, A. O. [1] ; Costa, J. M. [1] ; Oliveira, A. C. [1] ; Dias, M. A. D. [2, 3] ; Reis Neto, R. V. [4] ; Hilsdorf, A. W. S. [2, 3] ; Pinhal, D. [1]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Genet, BR-18618689 Botucatu, SP - Brazil
[2] Univ Mogi das Cruzes, Unit Biotechnol, BR-08780911 Mogi Das Cruzes, SP - Brazil
[3] Univ Fed Lavras, Dept Anim Sci, BR-37200000 Lavras, MG - Brazil
[4] Sao Paulo State Univ UNESP, BR-11900000 Registro, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: ANIMAL GENETICS; v. 51, n. 4 MAY 2020.
Web of Science Citations: 0
Abstract

The Nile tilapia (Oreochromis niloticus) is a prominent farmed fish in aquaculture worldwide. Crossbreeding has recently been carried out between the Red-Stirling and the wt Chitralada strains of Nile tilapia, producing a heterotic hybrid (7/8 Chitralada and 1/8 Red-Stirling) that combines the superior growth performance of the Chitralada with the reddish coloration of the Red-Stirling strain. While classical selective breeding and crossbreeding strategies are well known, the molecular mechanisms underlying the phenotypic expression of economically advantageous traits in tilapia remain largely unknown. Molecular investigations have shown that variable expression of growth hormone (gh), insulin-like growth factors (igf1 and 2) and somatolactin (smtla) - components of the growth hormone/insulin-like growth factor (GH/IGF) axis - and myostatin (mstn) genes can affect traits of economic relevance in farmed animals. The aim of this study was to assess and compare the gene expression signature among Chitralada, Red-Stirling and their backcross hybrid in order to gain insights into the effects of introgressive breeding in modulation of the GH/IGF axis. Gene expression analyses in distinct tissues showed that most genes of the GH/IGF axis were up-regulated and mstn was down-regulated in backcross animals in comparison with Red-Stirling and Chitralada animals. These gene expression profiles revealed that backcross animals displayed a distinctive expression signature, which attests to the effectiveness of the introgressive breeding technique. Our findings also suggest that the GH/IGF axis and mstn genes might be candidate markers for fish performance and prove useful within genetic improvement programs aimed at the production of superior-quality tilapia strains using introgressive breeding. (AU)

FAPESP's process: 14/03062-0 - Molecular characterization of heterotic hybrid from Red Stirling and Chitralada lines of Nile Tilapia (Oreochromis niloticus)
Grantee:Marcos Edgar Herkenhoff
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/15589-7 - Investigation of the composition, function and evolutionary dynamics of microRNAs in fish genomes using RNA-Seq and reverse genetics
Grantee:Danillo Pinhal
Support type: Regular Research Grants