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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antifungal compounds with anticancer potential from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa

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Grigoletto, Diana F. [1] ; Trivella, Daniela B. B. [2] ; Tempone, Andre G. [3] ; Rodrigues, Andre [4, 5] ; Correia, Ana Maria L. [4] ; Lira, Simone P. [1]
Total Authors: 6
[1] Univ Sao Paulo, Dept Ciencias Exatas, Escola Super Agr Luiz de Queiroz, CP 9, BR-13418900 Piracicaba, SP - Brazil
[2] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Biociencias, CP 6192, BR-13083970 Campinas, SP - Brazil
[3] Adolfo Lutz Inst, Centm Parasitol & Micol, BR-01246000 Sao Paulo, SP - Brazil
[4] Univ Estadual Paulista, UNESP, Dept Biol Geral & Aplicada, BR-13506900 Rio Claro, SP - Brazil
[5] Univ Estadual Paulista, UNESP, Ctr Estudos Insetos Sociais, BR-13506900 Rio Claro, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Brazilian Journal of Microbiology; v. 51, n. 3 APR 2020.
Web of Science Citations: 0

Fungi in the genus Trichoderma are notorious producers of secondary metabolites with diverse applications, such as antibacterial, antifungal, and plant growth-promoting properties. Peptaibols are linear peptides produced by such fungi, with more than 440 compounds described to date, including tricholongins, longibrachins, trichobrachins, and trichovirins. Peptaibols are synthesized by non-ribosomal peptide synthetases and they have several biological activities. Our research group isolated four peptaibols (6DP2, 6DP3, 6DP4, and 6DP5) with antifungal activity against the plant pathogen Colletotrichum gloeosporioides and the proteasome (a cancer chemotherapy target) from Trichoderma sp. P8BDA1F1, an endophytic fungus from Begonia venosa. The ethyl acetate extract of this endophyte showed activity of 6.01% and 75% against C. gloeosporioides and the proteasome, respectively. The isolated compounds were identified by MS/MS and compared to literature data, suggesting the presence of trilongins BI, BII, BIII, and BIV, which are peptaibols containing 20 amino acid residues. The minimum inhibitory concentration against C. gloeosporioides was 40 mu M for trilongin BI, 320 mu M for trilongin BII, 160 mu M for trilongin BIII, and 310 mu M for trilongin BIV. BI-BIV trilongins inhibited proteasome ChTL activity, with IC50 values of 6.5 +/- 2.7; 4.7 +/- 1.8; 6.3 +/- 2.2; and 2.7 +/- 0.5 mu M, respectively. The compounds were tested ex vivo against the intracellular amastigotes of Leishmania (L.) infantum but showed no selectivity. It is the first report of trilongins BI-BIV with antifungal activity against C. gloeosporioides and the proteasome target. (AU)

FAPESP's process: 14/10753-9 - Bioprospecting and rational design for the discovery of next generation proteasome inhibitors
Grantee:Daniela Barretto Barbosa Trivella
Support Opportunities: Regular Research Grants
FAPESP's process: 14/15760-3 - Biotechnological potential of secondary metabolites from plants and endophytic fungi
Grantee:Simone Possedente de Lira
Support Opportunities: Regular Research Grants
FAPESP's process: 13/50228-8 - Biodiversity components, and their metabolic characters, of Brazilian Islands: an integrated approach
Grantee:Roberto Gomes de Souza Berlinck
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 14/12021-5 - Endophytic fungi from Begonia sp. in Alcatrazes Island and the continental zone: diversity and prospection for bioactive compounds
Grantee:Ana Maria Lima Correia
Support Opportunities: Scholarships in Brazil - Master