Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fibrolytic enzymes improving in vitro rumen degradability of tropical forages

Full text
Sakita, Gabriel Zanuto [1] ; Ventoso Bompadre, Thiago Francisco [1] ; Dineshkumar, Dhanasekaran [1] ; Tavares Lima, Paulo de Mello [1] ; Abdalla Filho, Adibe Luiz [2] ; Campioni, Tania Sila [3] ; de Oliva Neto, Pedro [3] ; Bremer Neto, Herman [4] ; Louvandini, Helder [1] ; Abdalla, Adibe Luiz [1]
Total Authors: 10
[1] Univ Sao Paulo, Ctr Energia Nucl Agr, Lab Nutr Anim, Piracicaba, SP - Brazil
[2] Univ Sao Paulo, Ctr Energia Nucl Agr, Lab Ecol Isotop, Av Centenario 303, BR-13400970 Piracicaba, SP - Brazil
[3] Univ Estadual Paulista, Unidade Bioproc, Inst Pesquisa Bioenergia, Assis, SP - Brazil
[4] Univ Oeste Paulista, Lab Biofis, Dept Ciencias Funcionais, Presidente Prudente, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Web of Science Citations: 0

During specific times of the year, especially dry seasons, tropical forages typically have poor nutritional value due to high contents of neutral and acid detergent fibres, and low crude protein in their composition, which may reduce productivity of ruminant livestock production and lead to increased enteric methane (CH4) emissions per unit of generated product in forage-bases systems. In order to increase fibre degradability and the efficiency of energy utilisation from low-quality forages, exogenous fibrolytic enzymes have been studied. In this assay, we evaluated the effects of increasing dose levels of fibrolytic enzymes extract (FEE) produced by Trichoderma reesei on in vitro rumen organic matter degradability, fermentation parameters, total gas and CH4 production of tropical forages. Forage samples were analysed for their bromatological composition, and enzyme activity from FEE was performed for xylanase and endoglucanase. The in vitro gas production technique was used in a 5 x 3 factorial arrangement with five FEE dose levels (0, 5, 50, 500 and 5,000 mu l) and three substrates (Cynodon spp., Panicum maximum and Cenchrus ciliaris L.). The highest dose level of FEE increased degradability, total gas and CH4 production in all substrates (p < .05). Butyrate concentration also increased while acetate:propionate ratio and pH decreased with the addition of FEE (p < .05). These results indicated that the use of fibrolytic enzymes can be a reliable strategy to improve degradability of low-quality forages, contributing to the sustainability and intensification of livestock production in tropical countries. (AU)

FAPESP's process: 14/24188-1 - Development of bioprocesses for obtention of products with potential applications in bioenergy and feed industries using agro-industrial residues
Grantee:Pedro de Oliva Neto
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants