| Full text | |
| Author(s): Show less - |
Carvalho, Karen F.
[1]
;
Machado, Thiago S.
[2]
;
Garcia, Bruna M.
[1]
;
Zangirolamo, Amanda F.
[3]
;
Macabelli, Carolina H.
[1]
;
Sugiyama, Fabricia H. C.
[1]
;
Grejo, Mateus P.
[1]
;
Neto, J. Djaci Augusto
[1]
;
Tostes, Katiane
[1]
;
Ribeiro, Fernanda K. S.
[1]
;
Sarapiao, Fabiana D.
[3]
;
Pandey, Anand K.
[1, 4]
;
Nociti, Ricardo P.
[5]
;
Tizioto, Polyana
[6]
;
Coutinho, Luiz Lehman
[7]
;
Meirelles, Flavio V.
[2, 5]
;
Guimaraes, Francisco E. G.
[8]
;
Pernas, Lena
[9]
;
Seneda, Marcelo M.
[3]
;
Chiaratti, Marcos R.
[2, 1]
Total Authors: 20
|
| Affiliation: | [1] Univ Fed Sao Carlos, Departamento Genet & Evolucao, Rod Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Sao Paulo - Brazil
[3] Univ Estadual Londrina, Natl Inst Sci & Technol Dairy Prod Chain INCT LEI, Londrina, Parana - Brazil
[4] Lala Lajpat Rai Univ Vet & Anim Sci, Coll et Sci, Hisar, Haryana - India
[5] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Pirassununga - Brazil
[6] NGS Solucoes Genom, Piracicaba - Brazil
[7] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Piracicaba - Brazil
[8] Univ Sao Paulo, Inst Fis Sao Carlos, Sao Carlos - Brazil
[9] Max Planck Inst Biol Ageing, Cologne - Germany
Total Affiliations: 9
|
| Document type: | Journal article |
| Source: | FASEB JOURNAL; v. 34, n. 6 APR 2020. |
| Web of Science Citations: | 1 |
| Abstract | |
Mitochondrial function, largely regulated by the dynamics of this organelle, is inextricably linked to the oocyte health. In comparison with most somatic cells, mitochondria in oocytes are smaller and rounder in appearance, suggesting limited fusion. The functional implications of this distinct morphology, and how changes in the mitochondrial shape translate to mitochondrial function in oogenesis is little understood. We, therefore, asked whether the pro-fusion proteins mitofusins 1 (MFN1) and 2 (MFN2) are required for the oocyte development. Here we show that oocyte-specific deletion of Mfn1, but not Mfn2, prevents the oocyte growth and ovulation due to a block in folliculogenesis. We pinpoint the loss of oocyte growth and ovulation to impaired PI3K-Akt signaling and disrupted oocyte-somatic cell communication. In support, the double loss of Mfn1 and Mfn2 partially rescues the impaired PI3K-Akt signaling and defects in oocyte development secondary to the single loss of Mfn1. Together, this work demonstrates that the mitochondrial function influences the cellular signaling during the oocyte development, and highlights the importance of distinct, nonredundant roles of MFN1 and MFN2 in oogenesis. (AU) | |
| FAPESP's process: | 12/50231-6 - Molecular basis of mitochondrial inheritance: the role of mitochondrial fusion |
| Grantee: | Marcos Roberto Chiaratti |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 16/11935-9 - Effect of the knockout of mitofusin 2 on mitochondria, endoplasmic reticulum and mitophagy in murine oocytes |
| Grantee: | Bruna Martins Garcia |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials |
| Grantee: | Igor Polikarpov |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 13/08135-2 - CTC - Center for Cell-Based Therapy |
| Grantee: | Dimas Tadeu Covas |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 17/04372-0 - Mitochondrial DNA: mechanisms for genome integrity maintenance and impact on disease |
| Grantee: | Nadja Cristhina de Souza Pinto |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/11942-5 - Fertility effect of the knockout of Mitofusin 1 on murine oocytes |
| Grantee: | Karen Freire Carvalho |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 16/07868-4 - Effect of the mitofusins knockout on the inheritance of deleterious mitochondrial DNA in mouse embryonic fibroblasts |
| Grantee: | Carolina Habermann Macabelli |
| Support Opportunities: | Scholarships in Brazil - Doctorate |