Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biomarkers of the oxidative stress and neurotoxicity in tissues of the bullfrog, Lithobates catesbeianus to assess exposure to metals

Full text
Carvalho, Cleoni dos Santos [1, 2] ; Moraes Utsunomiya, Heidi Samantha [1, 2] ; Pasquoto-Stigliani, Tatiane [3] ; Costa, Monica Jones [1, 2] ; Fernandes, Marisa Narciso [4]
Total Authors: 5
[1] Univ Fed Sao Carlos, Campus Sorocaba, Rodovia Joao Leme dos Santos, BR-18052780 Sorocaba, SP - Brazil
[2] Univ Fed Sao Carlos, Programa Posgrad Biotecnol & Monitoramento Ambien, Campus Sorocaba, Rodovia Joao Leme dos Santos, BR-18052780 Sorocaba, SP - Brazil
[3] Univ Sorocaba, Programa Posgrad Ciencias Farmaceut, Rodovia Raposo Tavares, Km 92, 5 Artura, BR-18023000 Sorocaba, SP - Brazil
[4] Univ Fed Sao Carlos, Dept Ciencias Fisiol, Caixa Postal 676, Rodovia Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Web of Science Citations: 1

Lithobates catesbeianus tadpoles were exposed to 1 mu g L-1 of zinc (Zn), copper (Cu) and cadmium (Cd) alone or combined (1:1 and 1:1:1) for 2 and 16 days. Results showed a significant increase in the superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST) activities in the liver, kidney and muscle (except for GPx) in the groups exposed to metal either alone or co-exposed after 2 days compared to the control. After 16 days, SOD, CAT and GST activities decreased significantly in the liver and kidney and GPx activity increased in the liver. Reduced glutathione (GSH) increased in the liver and kidney following combined exposure and decreased after 2 days of metal exposure in the muscle. There were significant increases in lipid hydroperoxide (LPO) levels in the liver, kidney and muscle (2 and 16 days), with the highest levels after metal co-exposure. Cholinesterase (ChE) activity increased significantly in the brain after 2 days of exposure but decreased in the brain (16 days) and muscle (2 days) after exposure to metals, alone and combined. The current study highlighted that the antioxidant system of L. catesbeianus was sensitive to metals and specially to the co-exposure of the three metals, despite presenting differences in the response among tissues. In addition, tadpoles were sensitive at both periods of exposure, but in different modes with stress response (activation, upregulation) at 2 days and oppression (down-regulation) at 16 days. (AU)

FAPESP's process: 17/23781-9 - Ecotoxicological evaluation of water from the Sorocaba river using an integrated analysis of biochemical and morphological biomarkers in bullfrog tadpoles, Lithobates catesbeianus (Shaw, 1802).
Grantee:Cleoni dos Santos Carvalho
Support Opportunities: Regular Research Grants