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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evidence that Melatonin Increases Inhibin Beta-A and Follistatin Gene Expression in Ovaries of Pinealectomized Rats

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Author(s):
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Maganhin, Carla C. [1] ; Baracat, Maria Candida P. [1] ; Carvalho, Katia C. [1] ; Seganfredo, Isadora Braga [1] ; Luquetti, Camilla Maganhin [1] ; Simoes, Ricardo Dos Santos [1] ; Ferraz Carbonel, Adriana Aparecida [2] ; Simoes, Manuel de Jesus [2] ; Cipolla-Neto, Jose [3] ; Batista Castello Girao, Manoel Joao [4] ; Baracat, Edmund C. [1] ; Soares-Jr, Jose M.
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Hosp Clin, Dept Obstet & Ginecol, Lab Invest Med LIM 58, Disciplina Ginecol, Fac Med, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Morfol & Genet, Escola Paulista Med, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Fisiol & Biofis, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Dept Ginecol, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: REPRODUCTIVE SCIENCES; v. 27, n. 7 FEB 2020.
Web of Science Citations: 0
Abstract

Melatonin plays an important role in the regulation of ovarian function including oocyte maturation in different mammalian species. Many studies indicate that melatonin has an impact on the ovarian function of a variety of ovarian cells. However, the information on the exact mechanism and involved hormones is low. To evaluate inhibin beta-A (INHBA) and follistatin (FST) expression in the ovaries of pinealectomized rats treated with melatonin, thirty adult female Wistar rats were randomized into three groups of ten animals each: group 1 (GSh), sham-operated controls receiving vehicle; group 2 (GPx), pinealectomized animals receiving vehicle; and group 3 (GPxMe), pinealectomized animals receiving replacement melatonin (1.0 mg/kg body weight. It was assumed that each animal drank 6.5 +/- 1.2 ml per night and weighs approximately 300 g.) for 60 consecutive days. The ovaries were collected for mRNA abundance and protein of INHBA and FST by qRT-PCR and immunohistochemical analyses, respectively. Treatment with melatonin resulted in the upregulation of INHBA and FST genes in the ovarian tissue of the melatonin-treated animals (GPxMe), when compared with GPx. These findings were then confirmed by analyzing the expression of protein by immunohistochemical analyses, which revealed higher immunoreactivity of INHBA and FST in GPxMe animals in the follicular cells compared with GSh and GPx rats. Melatonin increases the expression of INHBA and FST in the ovaries of pinealectomized female rats. (AU)

FAPESP's process: 11/51581-8 - Analysis of melatonina in women with infertility and submitted to program of in vitro fertilization: functional study in granulosa cells
Grantee:Carla Cristina Maganhin
Support Opportunities: Scholarships in Brazil - Post-Doctorate