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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development of novel dental restorative composites with dibasic calcium phosphate loaded chitosan fillers

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Tanaka, Carina B. [1] ; Lopes, Diana P. [2] ; Kikuchi, Lucia N. T. [2] ; Moreira, Maria Stella [2] ; Catalani, Luiz H. [3] ; Braga, Roberto R. [4] ; Kruzic, Jamie J. [1] ; Goncalves, Flavia [2]
Total Authors: 8
[1] UNSW Sydney, Sch Mech & Mfg Engn, Sydney, NSW 2052 - Australia
[2] Univ Ibirapuera, Ave Interlagos 1329, BR-04661100 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Quim, Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Odontol, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Dental Materials; v. 36, n. 4, p. 551-559, APR 2020.
Web of Science Citations: 0

The incorporation of antimicrobial agents in restorative dental composites has the potential to slow the development of carious lesions. Objective. The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility. Methods. Chitosan and chitosan/DCPA particles were synthetized by the electrospray method. Experimental composites were formulated by adding 0, 0.5, or 1.0 wt% particles into a resin matrix along with 60 wt% barium glass. The degree of conversion and mechanical properties were measured after 1 and 90 days of aging in water after photoactivation. Cytotoxicity and genotoxicity were evaluated using fibroblasts from dental pulp in conditioned medium. The antimicrobial activity against Streptococcus mutans was assessed by crystal violet biofilm assay. Results. The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 +/- 8.0% (p = 0.328) and 3.0 +/- 0.8% micronucleus per group (p = 0.1078), respectively. The antimicrobial results showed that all composites with approximately 20% less biofilm (p < 0.001) relative to the control. No chitosan release was detected from the composites, suggesting direct contact of the bacteria with exposed chitosan particles on the surface was responsible for the observed antimicrobial effect. The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water. Significance. It can be concluded that the addition of chitosan or chitosan/DCPA particles in the restorative composites induced antimicrobial activity without compromising the mechanical properties or biocompatibility of the composites. (C) 2020 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 16/13114-2 - Development of new bioactive restorative composites associated to nanoespheres of chitosan loaded with calcium phosphate
Grantee:Flavia Gonçalves
Support Opportunities: Regular Research Grants