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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Experimental models and methods for cutaneous wound healing assessment

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Masson-Meyers, Daniela S. [1, 2] ; Andrade, Thiago A. M. [1, 3] ; Caetano, Guilherme F. [1, 3] ; Guimaraes, Francielle R. [1, 4] ; Leite, Marcel N. [1] ; Leite, Saulo N. [1, 5] ; Frade, Marco Andrey C. [1]
Total Authors: 7
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, Div Dermatol, Ribeirao Preto, SP - Brazil
[2] Marquette Univ, Sch Dent, 1801 West Wisconsin Ave, Milwaukee, WI 53233 - USA
[3] Univ Ctr Herminio Ometto Fdn FHO, Grad Program Biomed Sci, Araras, SP - Brazil
[4] Univ Ctr Associated Sch Educ UNIFAE, Sao Joao Da Boa Vista, SP - Brazil
[5] Univ Ctr Educ Fdn Guaxupe UNIFEG, Guaxupe, MG - Brazil
Total Affiliations: 5
Document type: Review article
Source: International Journal of Experimental Pathology; v. 101, n. 1-2 MAR 2020.
Web of Science Citations: 0

Wound healing studies are intricate, mainly because of the multifaceted nature of the wound environment and the complexity of the healing process, which integrates a variety of cells and repair phases, including inflammation, proliferation, reepithelialization and remodelling. There are a variety of possible preclinical models, such as in mice, rabbits and pigs, which can be used to mimic acute or impaired for example, diabetic and nutrition-related wounds. These can be induced by many different techniques, with excision or incision being the most common. After determining a suitable model for a study, investigators need to select appropriate and reproducible methods that will allow the monitoring of the wound progression over time. The assessment can be performed by non-invasive protocols such as wound tracing, photographic documentation (including image analysis), biophysical techniques and/or by invasive protocols that will require wound biopsies. In this article, we provide an overview of some of the most often needed and used: (a) preclinical/animal models including incisional, excisional, burn and impaired wounds; (b) methods to evaluate the healing progression such as wound healing rate, wound analysis by image, biophysical assessment, histopathological, immunological and biochemical assays. The aim is to help researchers during the design and execution of their wound healing studies. (AU)

FAPESP's process: 14/23662-1 - Production of 3D scaffolds containing different concentrations of graphene, hydroxyapatite and tricalcium phosphate surface coated with human bone morphogenetic and P-1 latex proteins
Grantee:Guilherme Ferreira Caetano
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 16/16437-7 - Assessment of the viability of human skin ex vivo model (hOSEC) and its application efficacy studies of sunscreens in long-term photoaging
Grantee:Marco Andrey Cipriani Frade
Support Opportunities: Regular Research Grants