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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fidelity of DNA polymerases in the detection of intraindividual variation of mitochondrial DNA

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Ricardo, Paulo Cseri [1] ; Francoso, Elaine [1] ; Arias, Maria Cristina [1]
Total Authors: 3
[1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, Rua Mato 277, BR-05508090 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: MITOCHONDRIAL DNA PART B-RESOURCES; v. 5, n. 1, p. 108-112, 2020.
Web of Science Citations: 0

Here we investigated the consequences of PCR amplification errors in the identification of intraindividual mtDNA variation. The bumblebee Bombus morio was chosen as model for the COI gene amplification tests with two DNA polymerases (Taq and Q5) presenting different error rates. The amplifications using Taq resulted in a significant increase of singleton haplotypes per individual in comparison to Q5. The sequence characteristics indicated that Taq resulted haplotypes are mostly due to amplification errors. Studies focusing on intraindividual variability should address special attention to the DNA polymerase fidelity to avoid overestimation of heteroplasmic haplotypes. (AU)

FAPESP's process: 16/24669-5 - Genomics and evolutionary studies on bees
Grantee:Maria Cristina Arias
Support Opportunities: Regular Research Grants
FAPESP's process: 13/12530-4 - Population, evolutionary and genomic studies in bees
Grantee:Maria Cristina Arias
Support Opportunities: Regular Research Grants