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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Short communication: Characterization of the milk protein expression profiles in dairy buffaloes with and without subclinical mastitis

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Author(s):
Tanamati, Fernanda [1] ; Taylor, Jeremy F. [2] ; Behura, Susanta K. [2] ; Santos, Daniel J. A. [3] ; Stafuzza, Nedenia B. [4] ; Andrade, Willian B. F. [1] ; Gasparino, Eliane [5] ; Tonhati, Humberto [1]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ FCAV UNESP, Dept Anim Sci, BR-14884900 Jaboticabal - Brazil
[2] Univ Missouri, Div Anim Sci, Columbia, MO 65211 - USA
[3] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 - USA
[4] Inst Anim Sci IZ, BR-14174000 Sertaozinho - Brazil
[5] Maringa State Univ UEM, Dept Anim Sci, BR-87020900 Maringa, Parana - Brazil
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF DAIRY SCIENCE; v. 103, n. 3, p. 2677-2684, MAR 2020.
Web of Science Citations: 0
Abstract

The aim of this study was to characterize the proteins present in milk whey from buffaloes with and without subclinical mastitis using a proteomic approach to identify differentially expressed proteins as potential biomarkers for this disease. Whey from Murrah buffaloes with subclinical mastitis was compared with whey from healthy animals using liquid chromatography-tandem mass spectrometry. The annotated protein databases for Bubalus bubalis and .Bos taurus were used in the analysis, and the gene annotations from the buffalo and bovine reference assemblies were also used. After integrating gene annotations from both buffaloes and bovines, a total of 1,033 proteins were identified, of which 156 were differentially expressed. Eighteen biological processes were annotated with Gene Ontology. Cathelicidin-3 was identified as a potential biomarker for subclinical mastitis. These results are important to the characterization of mastitis in the buffalo mammary gland and may aid in the development of tools for early diagnosis. (AU)

FAPESP's process: 16/10526-8 - Characterization of expression profiles of milk proteins in healthy and subclinical mastitis affected dairy buffaloes
Grantee:Fernanda Tanamati
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 14/19321-4 - Study of the expression of genes related to mastitis in dairy buffaloes
Grantee:Fernanda Tanamati
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/25309-7 - Polymorphims and expression of the immune system genes associated with mastitis in dairy buffaloes
Grantee:Humberto Tonhati
Support type: Regular Research Grants