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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biological characterization of the UW402, UW473, ONS-76 and DAOY pediatric medulloblastoma cell lines

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Bonfim-Silva, Ricardo [1] ; Salomao, Karina Bezerra [1] ; Costa de Andrade Pimentel, Thais Valeria [2] ; Branquinho de Oliveira Menezes, Camila Cristina [3] ; Bonini Palma, Patricia Vianna [3] ; Fontes, Aparecida Maria [1]
Total Authors: 6
[1] Univ Sao Paulo, Dept Genet, Ribeirao Preto Med Sch, Ave Bandeirantes, 3900 Monte Alegre, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Clin Med, Ribeirao Preto Med Sch, Ave Bandeirantes, 3900 Monte Alegre, BR-14049900 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Blood Ctr, Clin Hosp, Ribeirao Preto Med Sch, Ave Tenente Catao Roxo, 2501 Monte Alegre, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Cytotechnology; v. 71, n. 5, p. 893-903, OCT 2019.
Web of Science Citations: 2

Medulloblastoma (MB) is the most common malignant brain tumor in children. Recent advances in molecular technologies allowed to classify MB in 4 major molecular subgroups: WNT, SHH, Group 3 and Group 4. In cancer research, cancer cell lines are important for examining and manipulating molecular and cellular process. However, it is important to know the characteristics of each cancer cell line prior to use, because there are some differences among them, even if they originate from the same cancer type. This study aimed to evaluate the similarities and differences among four human medulloblastoma cell lines, UW402, UW473, DAOY and ONS-76. The medulloblastoma cell lines were analyzed for (1) cell morphology, (2) immunophenotyping by flow cytometry for some specifics surface proteins, (3) expression level of adhesion molecules by RT-qPCR, (4) proliferative potential, (5) cell migration, and (6) in vivo tumorigenic potential. It was observed a relationship between cell growth and CDH1 (E-chaderin) adhesion molecule expression and all MB cell lines showed higher levels of CDH2 (N-chaderin) when compared to other adhesion molecule. ONS-76 showed higher gene expression of CDH5 (VE-chaderin) and higher percentage of CD144/VE-chaderin positive cells when compared to other MB cell lines. All MB cell lines showed low percentage of CD34, CD45, CD31, CD133 positive cells and high percentage of CD44, CD105, CD106 and CD29 positive cells. The DAOY cell line showed the highest migration potential, the ONS-76 cell line showed the highest proliferative potential and only DAOY and ONS-76 cell lines showed tumorigenic potential in vivo. MB cell lines showed functional and molecular differences among them, which it should be considered by the researchers in choosing the most suitable cellular model according to the study proposal. (AU)

FAPESP's process: 11/20829-4 - Epigenetic mechanisms of HOX gene modulation in medulloblastoma
Grantee:Aparecida Maria Fontes
Support Opportunities: Regular Research Grants
FAPESP's process: 11/18664-7 - The role of lncRNA Evf-2 in the modulation of Hox genes and metastatic potential of Medulloblastoma
Grantee:Ricardo Bonfim Silva
Support Opportunities: Scholarships in Brazil - Doctorate