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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Training counteracts DEX-induced microvascular rarefaction by improving the balance between apoptotic and angiogenic proteins

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Author(s):
Jesus, Isley [1, 2] ; Herrera, Naiara A. [1] ; Andreo, Jesus C. [3] ; Santos, Carlos F. [3] ; Amaral, Sandra L. [4, 1]
Total Authors: 5
Affiliation:
[1] PIPGCF UFSCar UNESP, Joint Grad Program Physiol Sci, Rodovia Washington Luiz, Km 235 Monjolinho 676, Sao Carlos, SP - Brazil
[2] Univ Versailles St Quentin En Yvelines, INSERM, U1179, UFR SSSV, Montigny Le Bretonneux - France
[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil
[4] Sao Paulo State Univ UNESP, Sci Fac, Dept Phys Educ, Av Engn Luiz Edmundo Carrijo Coube 14-01, Bauru, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Steroids; v. 156, APR 2020.
Web of Science Citations: 0
Abstract

This work investigated the mechanisms induced by exercise training that may contribute to attenuate dexamethasone (DEX)-induced microvascular rarefaction and hypertension. Wistar rats underwent training protocol or were kept sedentary for 8 weeks. Dexamethasone was administered during the following 14-days and hemodynamic parameters were recorded at the end. Capillary density (CD) and capillary-to-fiber ratio (C:F ratio) were obtained in soleus muscle (SOL). Also, vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor-2 (VEGFR-2), endothelial nitric oxide synthase (eNOS), B-cell lymphoma 2 (Bcl-2), Bcl-2-like protein 4 (Bax), p-BAX and caspase-3 cleaved protein levels were analyzed. DEX treatment significantly increased blood pressure (+14%), which was associated with reduced C:F ratio ( - 41.0%) and CD ( - 43.1%). Reduction of vessel density was associated with decreased VEGF ( -15.6%), VEGFR-2 (-14.6%), Bcl-2 ( -18.4%), Bcl-2/Bax ratio (- 29.0%) and p-Bax/Bax ( - 25.4%), and also with increased caspase-3 cleaved protein level (25%). Training, on the other hand, prevented microvessels loss by mitigating all proteins changes induced by DEX. In addition, angiogenic and apoptotic proteins were significantly correlated with CD, which, in turn, was associated with blood pressure. Therefore, we may point out that exercise training is a good strategy to attenuate DEX-induced microvascular rarefaction in soleus muscle and this response involves a better balance between apoptotic and angiogenic proteins, which may contribute for the attenuation of hypertension. (AU)

FAPESP's process: 14/18177-7 - Effects of physical exercise on hypertension and oxidative stress in dexamethasone-treated rats
Grantee:Naiara Araújo Herrera
Support type: Scholarships in Brazil - Master
FAPESP's process: 14/23229-6 - Physical training effects on hypertension, arterial stiffness and muscle atrophy induced by dexamethasone: role of oxidative stress
Grantee:Sandra Lia do Amaral Cardoso
Support type: Regular Research Grants