Herrera, Naiara A.
Tardelli, Lidieli P.
Dionisio, Thiago J.
Shinohara, Andre L.
Santos, Carlos F.
Amaral, Sandra Lia
Total Authors: 7
 PIPGCF UFSCar UNESP, Joint Grad Program Physiol Sci, Rodovia Washington Luiz, Km 235 Monjolinho 676, BR-1356590 Sao Carlos, SP - Brazil
 Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil
 Sao Paulo State Univ, UNESP, Sch Sci, Dept Phys Educ, Av Eng Luiz Edmundo Carrijo Coube 14-01, BR-17033360 Bauru, SP - Brazil
Total Affiliations: 3
Molecular and Cellular Endocrinology;
APR 5 2020.
Web of Science Citations:
Microcirculation maintenance is associated with microRNAs. Nevertheless, the role of microRNAs induced by training in preventing dexamethasone (DEX)-induced microvascular rarefaction remains unknown. The study aim was to investigate if training-induced microRNAs are able to improve microcirculation proteins and prevent DEX-induced microvascular rarefaction. Rats underwent training for 8 weeks and then were treated with DEX (50 mu g/kg per day, s.c.) for 14 days. Arterial pressure was measured and tibialis anterior (TA) muscle was collected for analyses. DEX induced hypertension concomitantly with capillary density loss (CD, -23.9%) and decrease of VEGF (-43.0%), p-AKT/AKT (-39.6%) and Bcl-2 (-23.0%) and an increase in caspase-3-cleaved protein level (+34.0%) in TA muscle. Training upregulated microRNA-126 expression (+13.1%), prevented VEGF (+61.4%), p-AKT/AKT (+37.7%), Bcl-2 (+7.7%) decrease and caspase-3-cleaved (-23.1%) increase associated with CD (+54.7%) reduction and hypertension prevention. MiRNA-126 upregulation, induced by training, plays a role in controlling microcirculation, which may be a potential target against DEX-induced microvascular rarefaction. (AU)