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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of Serological and Molecular Tests Used for the Identification of Toxoplasma gondii Infection in Patients Treated in an Ophthalmology Clinic of a Public Health Service in Sao Paulo State, Brazil

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Author(s):
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Murata, Fernando Henrique Antunes [1, 2, 3] ; Previato, Mariana [1, 2] ; Frederico, Fabio Batista [2, 4] ; Barbosa, Amanda Pires [4] ; Nakashima, Fabiana [1, 5] ; de Faria, Jr., Geraldo Magela [1, 2] ; Silveira Carvalho, Aparecida Perpetuo [1, 2] ; Meira Strejevitch, Cristina da Silva [6] ; Pereira-Chioccola, Vera Lucia [6] ; Castiglioni, Lilian [1] ; de Mattos, Luiz Carlos [1, 2] ; Siqueira, Rubens Camargo [1, 2] ; Brandao de Mattos, Cinara Cassia [1, 2]
Total Authors: 13
Affiliation:
[1] Fac Med Sao Jose do Rio Preto, Sao Jose Do Rio Preto - Brazil
[2] FAMERP Toxoplasma Res Grp, Sao Jose Do Rio Preto - Brazil
[3] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD - USA
[4] Fundacao Fac Reg Med Sao Jose Do Rio Preto, Ambulatorio Oftalmol Hosp Base, Sao Jose Do Rio Preto - Brazil
[5] Univ Fed Roraima, Boa Vista, Parana - Brazil
[6] Adolfo Lutz Inst, Lab Biol Mol Parasitas & Fungos, Ctr Parasitol & Micol, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 9, FEB 7 2020.
Web of Science Citations: 4
Abstract

Ocular toxoplasmosis is one of the most common complications caused by the infection with the parasite Toxoplasma gondii. The risk of developing eye lesions and impaired vision is considered higher in Brazil than other countries. The clinical diagnosis is difficult and the use of sensitive and specific laboratorial methods can aid to the correct diagnosis of this infection. We compared serological methods ELISA and ELFA, and molecular cPCR, Nested PCR and qPCR for the diagnosis of T. gondii infection in groups of patients clinically evaluated with ocular diseases non-toxoplasma related (G1 = 185) and with lesions caused by toxoplasmosis (G2 = 164) in an Ophthalmology clinic in Brazil. Results were compared by the Kappa index, and sensitivity (S), specificity (E), positive predictive value (PPV), and negative (NPV) were calculated. Serologic methods were in agreement with ELISA more sensitive and ELFA more specific to characterize the acute and chronic infections while molecular methods were discrepant where qPCR presented higher sensitivity, however, lower specificity when compared to cPCR and Nested PCR. (AU)

FAPESP's process: 12/07716-9 - Serological and molecular investigation of Toxoplasma gondii in blood donors
Grantee:Luiz Carlos de Mattos
Support type: Regular Research Grants
FAPESP's process: 12/07750-2 - Serological and molecular investigation of Toxoplasma gondii in blood donors
Grantee:Fabiana Nakashima
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/05302-8 - Isolation and genetic characterization of Toxoplasma gondii in Myrmecophaga tridactyla (Linnaeus, 1758)
Grantee:Lilian Castiglioni
Support type: Regular Research Grants
FAPESP's process: 18/04709-8 - Study of Toxoplasma gondii extracellular vesicles modulating the immune system of infected hosts
Grantee:Vera Lúcia Pereira Chioccola
Support type: Regular Research Grants
FAPESP's process: 18/09448-8 - Expression of miRNAs in ocular Toxoplasmosis
Grantee:Geraldo Magela de Faria Junior
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/15879-8 - Toxoplasma gondii infection: serological and molecular characterization in patients attended in public health service (SUS)
Grantee:Fernando Henrique Antunes Murata
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/10050-5 - Serological, molecular and images in patients with active lesions or acute relapse of ocular infection by Toxoplasma gondii: a study follow-up
Grantee:Mariana Previato
Support type: Scholarships in Brazil - Scientific Initiation