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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Real-time redox monitoring of a nitrosyl ruthenium complex acting as NO-donor agent in a single A549 cancer cell with multiplex Fourier-transform infrared microscopy

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Rodrigues, Fernando Postalli [1] ; Macedo, Lucyano J. A. [2] ; Maximo, Leandro N. C. [1, 3] ; Sales, Fernanda C. P. F. [2] ; da Silva, Roberto S. [1] ; Crespilho, Frank N. [2]
Total Authors: 6
[1] Univ Sao Paulo, Dept Phys & Chem, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sao Carlos Inst Chem, BR-13560970 Sao Carlos, SP - Brazil
[3] Inst Fed Educ Ciencia & Tecnol Goiano, Dept Chem, Campus Urutai, BR-75790000 Urutai, Go - Brazil
Total Affiliations: 3
Document type: Journal article
Source: NITRIC OXIDE-BIOLOGY AND CHEMISTRY; v. 96, p. 29-34, MAR 1 2020.
Web of Science Citations: 0

Multiplex Fourier-transform infrared microscopy (FT-IR) helped to monitor trans-{[}Ru(NO) (NH3)(4) (isn)](3+) (I), uptake by A549 lung carcinoma cell, as well as the generation of its product, nitric oxide (NO), inside the cell. Chronoamperometry with NO-sensor and FT-IR showed that exogenous NADH and the A549 cell induced the NO release redox mechanism. Chemical imaging confirmed that (I) was taken up by the cell, and that its localization coincided with its consumption in the cellular environment within 15 min of exposure. The Ru-NO absorption band in the IR spectrum shifted from 1932 cm(-1), when NO was coordinated to Ru as [Ru-II-NO+](3+), to 1876 cm(-1), due the formation of reduced species [Ru-II-NO0](2+), a precursor of NO release. Futhermore, the mu FT-IR spectral profile demonstrated that, as a result of the NO action on the target, NO interacted with nucleic acids, which provided a biochemical response that is detectable in living cells. (AU)

FAPESP's process: 13/07829-0 - Evaluation of Electrochemical Properties of Glucose/O2 Implantable Biofuel Cell
Grantee:Fernanda Cristina Pena Ferreira Sales
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 16/12707-0 - Cytotoxicity and photo-cytotoxicity of new ruthenium-phthalocyanines compounds as nitric oxide and oxygen singlet producers in cancer cell lines. an innovative purpose for metal based drug
Grantee:Roberto Santana da Silva
Support Opportunities: Regular Research Grants
FAPESP's process: 15/16672-3 - Development of High Performance Bioelectrodes for Application in Miniaturized Biofuel Cell
Grantee:Frank Nelson Crespilho
Support Opportunities: Regular Research Grants
FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
Grantee:Osvaldo Novais de Oliveira Junior
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/20493-2 - Study of metalloenzymes through electrochemistry coupled to vibrational spectroscopy
Grantee:Lucyano Jefferson Alves de Macêdo
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/03964-3 - Ruthenium nitrosil complex as producer of nitric oxide and singlet oxygen: FOTOBIOLOGICAL studies, BICHEMICAL and their potential therapeutical aplication
Grantee:Fernando Postalli Rodrigues
Support Opportunities: Scholarships in Brazil - Post-Doctoral