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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Docosahexaenoic acid slows inflammation resolution and impairs the quality of healed skin tissue

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Author(s):
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Candreva, Thamiris [1] ; Kuhl, Carolina M. C. [1] ; Burger, Beatriz [1] ; dos Anjos, Mariah B. P. [1] ; Torsoni, Marcio A. [2] ; Consonni, Silvio R. [3] ; Crisma, Amanda R. [4] ; Fisk, Helena L. [5] ; Calder, Philip C. [6, 7, 5] ; de Mato, Felipe C. P. [8] ; Sernaglia, Erica M. [8] ; Vinolo, Marco A. R. [8] ; Rodrigues, Hosana G. [1]
Total Authors: 13
Affiliation:
[1] Univ Estadual Campinas, Sch Appl Sci, Lab Nutrients & Tissue Repair, Limeira, SP - Brazil
[2] Univ Estadual Campinas, Sch Appl Sci, Lab Metab Disorders, Limeira, SP - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
[4] Univ Sao Paulo, Dept Physiol & Biophys, Sao Paulo, SP - Brazil
[5] Univ Southampton, Human Dev & Hlth, Fac Med, Southampton, Hants - England
[6] Univ Southampton, Southampton, Hants - England
[7] Univ Hosp Southampton NHS Fdn Trust, NIHR Southampton Biomed Res Ctr, Southampton, Hants - England
[8] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Campinas, SP - Brazil
Total Affiliations: 8
Document type: Journal article
Source: Clinical Science; v. 133, n. 22, p. 2345-2360, NOV 2019.
Web of Science Citations: 0
Abstract

There is no consensus on the effects of omega-3 (omega-3) fatty acids (FA) on cutaneous repair. To solve this problem, we used 2 different approaches: (1) FAT-1 transgenic mice, capable of producing endogenous omega-3 FA; (2) wild-type (WT) mice orally supplemented with DHA-enriched fish oil. FAT-1 mice had higher systemic (serum) and local (skin tissue) omega-3 FA levels, mainly docosahexaenoic acid (DHA), in comparison with WT mice. FAT-1 mice had increased myeloperoxidase (MPO) activity and content of CXCL-1 and CXCL-2, and reduced IL-10 in the skin wound tissue three days after the wound induction. Inflammation was maintained by an elevated TNF-alpha concentration and presence of inflammatory cells and edema. Neutrophils and macrophages, isolated from FAT-1 mice, also produced increased TNF-alpha and reduced IL-10 levels. In these mice, the wound closure was delayed, with a wound area 6-fold bigger in relation with WT group, on the last day of analysis (14 days post-wounding). This was associated with poor orientation of collagen fibers and structural aspects in repaired tissue. Similarly, DHA group had a delay during late inflammatory phase. This group had increased TNF-alpha content and CD45(+)F4/80(+) cells at the third day after skin wounding and increased concentrations of important metabolites derived from omega-3, like 18-HEPE, and reduced concentrations of those from omega-6 FA. In conclusion, elevated DHA content, achieved in both FAT-1 and DHA groups, slowed inflammation resolution and impaired the quality of healed skin tissue. (AU)

FAPESP's process: 14/15127-9 - Multiuser equipment approved in grant 2013/06810-4: flow citometry BD Accury
Grantee:Hosana Gomes Rodrigues
Support type: Multi-user Equipment Program
FAPESP's process: 13/06810-4 - Mechanisms of action of omega-3 and omega-6 in the tissue repair process: neuro-immune focus
Grantee:Hosana Gomes Rodrigues
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 16/02021-3 - Wound healing in FAT -1 mice: involvement of anti- inflammatory cholinergic pathway
Grantee:Thamiris Candreva Robles
Support type: Scholarships in Brazil - Master
FAPESP's process: 16/23298-3 - Analysis of phosphatidylcholine lipid fraction in fat-1 animals in the context of wound healing
Grantee:Thamiris Candreva Robles
Support type: Scholarships abroad - Research Internship - Master's degree