Da Silva, Caroline Caetano
Fernandes, Bianca H. V.
Carvalho, Luciani R.
Total Authors: 6
 Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Discipline Endocrinol, Internal Med Dept, Av Dr Eneas Carvalho Aguiar 155, BR-05403000 Sao Paulo - Brazil
 Szent Istvan Univ, Dept Aquaculture, Pater Karoly U 1, H-2100 Godollo - Hungary
 Univ Sao Paulo, Fac Med, Diretoria Tecn Apoio Ensino & Pesquisa, Sao Paulo - Brazil
Total Affiliations: 3
JUN 1 2019.
Web of Science Citations:
We developed an easy, efficient, and cheap protocol for zebrafish sperm cryopreservation carried out on dry ice (20min) using simple composition solution (200mM glucose, 40mM KCl, 30mM Tris, pH=8.0). The average efficiency of the present cryopreserve method was between 10% and 20% (expressed as fertilization rate). The experiments were conducted and repeated at two different locations, in different countries, yielding very similar results, showing the reproducibility and applicability of the method. (AU)