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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

An Easy Method for Cryopreservation of Zebrafish (Danio rerio) Sperm

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Da Silva, Caroline Caetano [1] ; Kollar, Timea [2] ; Csenki-Bakos, Zsolt [2] ; Fernandes, Bianca H. V. [3] ; Horvath, Akos [2] ; Carvalho, Luciani R. [1]
Total Authors: 6
[1] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Discipline Endocrinol, Internal Med Dept, Av Dr Eneas Carvalho Aguiar 155, BR-05403000 Sao Paulo - Brazil
[2] Szent Istvan Univ, Dept Aquaculture, Pater Karoly U 1, H-2100 Godollo - Hungary
[3] Univ Sao Paulo, Fac Med, Diretoria Tecn Apoio Ensino & Pesquisa, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ZEBRAFISH; v. 16, n. 3, p. 321-323, JUN 1 2019.
Web of Science Citations: 0

We developed an easy, efficient, and cheap protocol for zebrafish sperm cryopreservation carried out on dry ice (20min) using simple composition solution (200mM glucose, 40mM KCl, 30mM Tris, pH=8.0). The average efficiency of the present cryopreserve method was between 10% and 20% (expressed as fertilization rate). The experiments were conducted and repeated at two different locations, in different countries, yielding very similar results, showing the reproducibility and applicability of the method. (AU)

FAPESP's process: 15/26563-7 - Molecular genetic diagnosis in patients with pituitary and gonadal developmental disorders and the use of models in vitro and in vivo to evaluate the functional effect of allelic variants identified by sequencing large scale
Grantee:Luciani Renata Silveira de Carvalho
Support Opportunities: Regular Research Grants