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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Unveiling resistome profiles in the sediments of an Antarctic volcanic island

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Author(s):
Centurion, V. B. [1, 2] ; Delforno, T. P. [1] ; Lacerda-Junior, G. V. [1, 3] ; Duarte, A. W. F. [1, 4] ; Silva, U. [3] ; Bellini, G. B. [1, 2] ; Rosa, L. H. [5] ; Oliveira, V. M. [1]
Total Authors: 8
Affiliation:
[1] State Univ Campinas UNICAMP, Res Ctr Chem Biol & Agr CPQBA, Microbial Resources Div, BR-13081970 Paulinia, SP - Brazil
[2] State Univ Campinas UNICAMP, Biol Inst, BR-13083862 Campinas, SP - Brazil
[3] Brazilian Agr Res Corp EMBRAPA, BR-13820000 Jaguariuna, SP - Brazil
[4] Univ Fed Alagoas, Campus Arapiraca UFAL, BR-57309005 Arapiraca, AL - Brazil
[5] Fed Univ Minas Gerais UFMG, Inst Biol Sci, BR-31270901 Belo Horizonte, MG - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Environmental Pollution; v. 255, n. 2 DEC 2019.
Web of Science Citations: 0
Abstract

The Deception Island, located in Maritime Antarctica, is a volcanic island with geothermal activity and one of the most visited by tourists. However, the extent of the anthropogenic impact remains largely unknown and the factors shaping the resistance/tolerance mechanisms in the microbiomes from Whalers Bay ecosystems have never been investigated. In this context, this study aimed to reveal the resistome profiles of Whalers Bay sediments and correlate them with environmental factors. Samples were collected at four sites during the summer 2014/2015 along a transect of 27.5 m in the Whalers Bay sediments. DNA isolated from sediment samples was sequenced using the Illumina HiSeq platform. Bioinformatic analyses allowed the assembly of contigs and scaffolds, prediction of ORFs, and taxonomic and functional annotation using NCBI RefSeq database and KEGG orthology, respectively. Microorganisms belonging to the genera Psychrobacter, Flavobacterium and Polaromonas were shown to dominate all sites, representing 60% of taxonomic annotation. Arsenic (As), copper (Cu) and iron (Fe) were the most abundant metal resistance/tolerance types found in the microbiomes. Beta-lactam was the most common class related to antibiotics resistance/tolerance, corroborating with previous environmental resistome studies. The acridine class was the most abundant amongst the biocide resistance/tolerances, related to antiseptic compounds. Results gathered in this study reveal a repertoire of resistance/tolerance classes to antibiotics and biocides unusually found in Antarctica. However, given the volcanic nature (heavy metals-rich region) of Deception Island soils, this putative impact must be viewed with caution. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 16/05640-6 - Multi-omics applied to the understanding and exploitation of Antarctic microbiomes
Grantee:Valeria Maia Merzel
Support type: Regular Research Grants
FAPESP's process: 17/03172-8 - Mechanisms of stress adaptation in sediment microbiome of the Deception Volcanic Island in the Maritime Antarctic
Grantee:Victor Borin Centurion Biruel
Support type: Scholarships in Brazil - Doctorate (Direct)