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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice

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Author(s):
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Portuondo, Deivys Leandro [1] ; Dores-Silva, Paulo Roberto [2] ; Ferreira, Lucas Souza [1] ; de Oliveira, Carlos S. [2] ; Tellez-Martinez, Damiana [1] ; Marcos, Caroline Maria [1] ; de Aguiar Loesch, Maria Luiza [1] ; Guzman, Fanny [3] ; Gava, Lisandra M. [4] ; Borges, Julio Cesar [2] ; Pereira, Sandro Antonio [5] ; Batista-Duharte, Alexander [1] ; Carlos, Iracilda Zeppone [1]
Total Authors: 13
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Clin Anal, Araraquara, SP - Brazil
[2] Univ Sao Paulo, Sao Carlos Inst Chem, POB 780, BR-13560970 Sao Carlos, SP - Brazil
[3] Pontificia Univ Catolica Valparaiso, NBC, Valparaiso - Chile
[4] Univ Fed Sao Carlos, Ctr Biol & Hlth Sci, BR-13560970 Sao Carlos, SP - Brazil
[5] Oswaldo Cruz Fdn Fiocruz, Evandro Chagas Natl Inst Infect Dis INI, Lab Clin Res Dermatozoonoses Domest Anim Lapclin, Rio De Janeiro, RJ - Brazil
Total Affiliations: 5
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, NOV 20 2019.
Web of Science Citations: 0
Abstract

In recent years, research has focused on the immunoreactive components of the Sporothrix schenckii cell wall that can be relevant targets for preventive and therapeutic vaccines against sporotrichosis, an emergent worldwide mycosis. In a previous study, we identified a 47-kDa enolase as an immunodominant antigen in mice vaccinated with an adjuvanted mixture of S. schenckii cell wall proteins. Here, we sought to assess the protective potential of a Sporothrix spp. recombinant enolase (rSsEno) formulated with or without the adjuvant Montanide Pet-GelA (PGA) against the S. brasiliensis infection in mice. Mice that were immunized with rSsEno plus PGA showed increased antibody titters against rSsEno and increased median survival time when challenged with S. brasiliensis as compared with mice that had not been immunized or that were immunized with rSsEno alone. Immunization with rSsEno plus PGA induced a predominantly T-helper 1 cytokine pattern after in vitro stimulation of splenic cells with rSsEno: elevated levels of IFN-gamma and IL-2, as well as of other cytokines involved in host defense against sporotrichosis, such as TNF-alpha, IL-6, and IL-4. Furthermore, we show for the first time the presence of enolase in the cell wall of both S. schenckii and S. brasiliensis. As a whole, our results suggest that enolase could be used as a potential antigenic target for vaccinal purposes against sporotrichosis. (AU)

FAPESP's process: 17/13228-0 - Evaluation of the amino acid modification, structural characterization and immunogenicity of S. schenckii enolase-derived P1-A and P3-A peptides for a sporotrichosis vaccine
Grantee:Deivys Leandro Portuondo Fuentes
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 17/26774-3 - Immunogenicity and preclinical efficacy of a recombinant enolase vaccine candidate against Sporothrix schenckii and Sporothrix brasiliensis
Grantee:Iracilda Zeppone Carlos
Support Opportunities: Regular Research Grants
FAPESP's process: 15/09340-4 - Identification and synthesis of enolase peptides Sporothrix schenckii as vaccine candidates in sporotrichosis
Grantee:Deivys Leandro Portuondo Fuentes
Support Opportunities: Scholarships in Brazil - Post-Doctorate